Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3446409..3447027 Replicon chromosome
Accession NZ_CP086520
Organism Escherichia coli strain Ec-042-T20-ESBL

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LNN45_RS16790 Protein ID WP_001291435.1
Coordinates 3446809..3447027 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LNN45_RS16785 Protein ID WP_000344800.1
Coordinates 3446409..3446783 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN45_RS16775 (3441498) 3441498..3442691 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN45_RS16780 (3442714) 3442714..3445863 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LNN45_RS16785 (3446409) 3446409..3446783 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LNN45_RS16790 (3446809) 3446809..3447027 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LNN45_RS16795 (3447199) 3447199..3447750 + 552 WP_000102568.1 maltose O-acetyltransferase -
LNN45_RS16800 (3447866) 3447866..3448336 + 471 WP_000136192.1 YlaC family protein -
LNN45_RS16805 (3448500) 3448500..3450050 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LNN45_RS16810 (3450092) 3450092..3450445 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LNN45_RS16820 (3450824) 3450824..3451135 + 312 WP_000409911.1 MGMT family protein -
LNN45_RS16825 (3451166) 3451166..3451738 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223679 WP_001291435.1 NZ_CP086520:3446809-3447027 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223679 NZ_CP119127:660334-660588 [Sphingobium sp. WTD-1]
ATGCAGACCGTCAGCGTTTCCGAAACCCGCGCCAATCTCAAGGCCGTGCTCGACCGTGTCGTCGCCGACAAGGCGCCGAT
CCAGATCGTGCGGCCCAAGGGTGAGGGCGTGGTGATGATTTCGCAAAGCGAATGGGAAGGCATGCAGGAGACGCTGCACC
TGCTGGCGTCGCCCGCCAATGCCCATCATCTGCTGGAGGGCATCAGGCAACTGGATGCCGGGCTCGGCGAGGAGCATGAG
CTGATCGCGCCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223679 WP_000344800.1 NZ_CP086520:3446409-3446783 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223679 NZ_CP119127:660585-660851 [Sphingobium sp. WTD-1]
GTGAAGATCGTCTTCGGGGGCCAGGCCTGGCGCGACTATGTCGCCTGGGCAGCGGATGATCCCAAGGTGCTGGCACGGAT
CAACGCGCTGATCGAGGAATGCCGCCGCGATCCGTTTCGCGGCACGGGCAAGCCGGAGCCATTGCGGCAGAACCTGGCCG
GCTGGTGGTCAAGGCGCATCACTGGCGAGCATCGGCTGGTCTATCGGGTCAGCGGATCAGGCGACACGCAGGCGCTGGAA
GTGCTGAGTTGCCGGTATCATTATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References