Detailed information of TA system TA223678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2356443..2358031
• Replicon: chromosome
• Accession: NZ_CP086520
• Organism: Escherichia coli strain Ec-042-T20-ESBL

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: LNN45_RS11430
• Protein ID: WP_001125463.1
• Coordinates: 2356709..2358031 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LNN45_RS11425
• Protein ID: WP_001296726.1
• Coordinates: 2356443..2356709 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN45_RS11425 (2356443)	2356443..2356709	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LNN45_RS11430 (2356709)	2356709..2358031	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LNN45_RS11435 (2358343)	2358343..2358551	+	209	Protein_2241	AraC family transcriptional regulator	-
LNN45_RS11440 (2358983)	2358983..2359546	+	564	WP_001373493.1	type 1 fimbrial major subunit FimA	-
LNN45_RS11445 (2359907)	2359907..2360170	+	264	Protein_2243	fimbria/pilus periplasmic chaperone	-
LNN45_RS11450 (2360172)	2360172..2361485	+	1314	Protein_2244	fimbrial biogenesis outer membrane usher protein	-
LNN45_RS11455 (2361499)	2361499..2362029	+	531	WP_000876784.1	fimbrial protein	-
LNN45_RS11460 (2362042)	2362042..2362545	+	504	WP_000825458.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2356443..2372042	15599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T223678 WP_001125463.1 NZ_CP086520:2356709-2358031 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T223678 NZ_CP119127:c300471-300289 [Sphingobium sp. WTD-1]
ATGGAACGGGACAGCAAGAAGATCGTCAAGCGGTTGCTCGAAGAGGGTTTCGAGCAGATTTCAGTGAGGGGCTCTCACCA
TAAGTTCCGCAAGGGCGCCGTGACGATCATCGTGCCGCACCCGAAGAAGGACCTTCCGATCGGCACCGCCCGGTCGATTG
CGAAGGCGGCGGGCTGGCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT223678 WP_001296726.1 NZ_CP086520:2356443-2356709 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT223678 NZ_CP119127:c300248-299856 [Sphingobium sp. WTD-1]
ATGAAATATTTCTATGCCTTGGTGCATAAGGATGCGGATAGCGCCTTCGGTGTCAGCTTCCCCGATCTGCCCGGCTGCTT
TTCTGCCGCCGATCGGCAGGAGGATGTTTTGGCCAACGCGGTCGAGGCGCTGGAACTCTGGTTCGAGGATGCCGACGCCG
TCGATCCAAGACCGGTCGACCAGGTGCGCGAAGCGGCAAAGGATGACCTGGCCGACGGCGCCTTCCTGCTCGCCGTCCCG
CATATCGTGTCGGCGAACAAGCTTATGCGCATCAACCTGTCGCTGGACCGGGGCATGCTCGATGCCATCGACCAGGCCGC
CGCACTGCGCAAATTGACCCGCAGTGCCTTCATGACGGAAGCCGCGCGCAATGAGATTCAGGGACGCCATTGA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0