Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 337538..338338 Replicon chromosome
Accession NZ_CP086520
Organism Escherichia coli strain Ec-042-T20-ESBL

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag LNN45_RS01575 Protein ID WP_000342449.1
Coordinates 337811..338338 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag LNN45_RS01570 Protein ID WP_001277108.1
Coordinates 337538..337804 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN45_RS01550 (333195) 333195..333863 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
LNN45_RS01555 (333856) 333856..334914 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
LNN45_RS01560 (335159) 335159..336013 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
LNN45_RS01565 (336285) 336285..337388 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
LNN45_RS01570 (337538) 337538..337804 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
LNN45_RS01575 (337811) 337811..338338 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
LNN45_RS01580 (338335) 338335..338718 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
LNN45_RS01585 (339142) 339142..340251 + 1110 WP_089455105.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
LNN45_RS01590 (340299) 340299..341225 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LNN45_RS01595 (341222) 341222..342499 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
LNN45_RS01600 (342496) 342496..343263 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T223663 WP_000342449.1 NZ_CP086520:337811-338338 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T223663 NZ_CP119120:2923491-2923865 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT223663 WP_001277108.1 NZ_CP086520:337538-337804 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT223663 NZ_CP119120:2923033-2923401 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References