Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 316247..316833 Replicon chromosome
Accession NZ_CP086520
Organism Escherichia coli strain Ec-042-T20-ESBL

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LNN45_RS01450 Protein ID WP_042014162.1
Coordinates 316247..316477 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LNN45_RS01455 Protein ID WP_000593555.1
Coordinates 316474..316833 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN45_RS01440 (312284) 312284..315019 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
LNN45_RS01445 (315019) 315019..316143 + 1125 WP_001314210.1 ABC transporter permease -
LNN45_RS01450 (316247) 316247..316477 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LNN45_RS01455 (316474) 316474..316833 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LNN45_RS01460 (316953) 316953..317354 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LNN45_RS01465 (317360) 317360..318166 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LNN45_RS01470 (318163) 318163..318927 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LNN45_RS01475 (318927) 318927..319760 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LNN45_RS01480 (319757) 319757..320701 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T223662 WP_042014162.1 NZ_CP086520:316247-316477 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T223662 NZ_CP119120:c2671408-2671305 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT223662 WP_000593555.1 NZ_CP086520:316474-316833 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT223662 NZ_CP119120:2671267-2671538 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References