223661

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 15031..15496 Replicon plasmid pEc-050-T0_6
Accession NZ_CP086516
Organism Escherichia coli strain Ec-050-T0

Toxin (Protein)


Gene name TsxA Uniprot ID A0A5Y6UAV0
Locus tag LNN50_RS25990 Protein ID WP_001230708.1
Coordinates 15031..15282 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag LNN50_RS25995 Protein ID WP_000801440.1
Coordinates 15269..15496 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN50_RS25970 (LNN50_25975) 12551..12886 + 336 WP_001147119.1 type II toxin-antitoxin system RelE/ParE family toxin -
LNN50_RS25975 (LNN50_25980) 12883..13233 + 351 WP_000127483.1 hypothetical protein -
LNN50_RS25980 (LNN50_25985) 13872..14387 + 516 WP_001025396.1 J domain-containing protein -
LNN50_RS25985 (LNN50_25990) 14492..15034 + 543 WP_001083908.1 hypothetical protein -
LNN50_RS25990 (LNN50_25995) 15031..15282 + 252 WP_001230708.1 hypothetical protein Toxin
LNN50_RS25995 (LNN50_26000) 15269..15496 + 228 WP_000801440.1 hypothetical protein Antitoxin
LNN50_RS26000 (LNN50_26005) 15888..16187 + 300 WP_000650313.1 hypothetical protein -
LNN50_RS26005 (LNN50_26010) 16279..16644 + 366 WP_000854262.1 hypothetical protein -
LNN50_RS26010 (LNN50_26015) 16919..17092 + 174 WP_071821486.1 type II toxin-antitoxin system HicB family antitoxin -
LNN50_RS26015 (LNN50_26020) 17455..17958 + 504 WP_000781818.1 DNA distortion polypeptide 1 -
LNN50_RS26020 (LNN50_26025) 17962..19191 + 1230 WP_000539545.1 DNA relaxase/nickase TaxC -
LNN50_RS26025 (LNN50_26030) 19268..19561 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
LNN50_RS26030 (LNN50_26035) 19558..19773 + 216 WP_000760375.1 EexN family lipoprotein -
LNN50_RS26035 (LNN50_26040) 19950..20126 + 177 WP_000660496.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33826 33826


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9750.19 Da        Isoelectric Point: 9.8104

>T223661 WP_001230708.1 NZ_CP086516:15031-15282 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T223661 NZ_CP119120:c2338392-2337070 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT223661 WP_000801440.1 NZ_CP086516:15269-15496 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT223661 NZ_CP119120:c2338658-2338392 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Y6UAV0

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References