Detailed information of TA system TA223659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 8292..8793
• Replicon: plasmid pEc-050-T0_5
• Accession: NZ_CP086515
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: LNN50_RS25580
• Protein ID: WP_000702246.1
• Coordinates: 8292..8552 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: LNN50_RS25585
• Protein ID: WP_001132407.1
• Coordinates: 8542..8793 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN50_RS25535 (LNN50_25540)	3826..3978	+	153	WP_001414491.1	DUF5431 family protein	-
LNN50_RS25540 (LNN50_25545)	3923..4045	+	123	WP_223685802.1	Hok/Gef family protein	-
LNN50_RS25545 (LNN50_25550)	4195..4791	+	597	WP_000125166.1	ProQ/FINO family protein	-
LNN50_RS25550 (LNN50_25555)	4809..5093	-	285	WP_001162371.1	hypothetical protein	-
LNN50_RS25555 (LNN50_25560)	5172..5837	-	666	WP_000532148.1	division plane positioning ATPase MipZ	-
LNN50_RS25560 (LNN50_25565)	6435..6764	+	330	WP_000855118.1	hypothetical protein	-
LNN50_RS25565 (LNN50_25570)	7345..7668	+	324	WP_000427402.1	hypothetical protein	-
LNN50_RS25570 (LNN50_25575)	7710..7919	+	210	WP_000866037.1	hypothetical protein	-
LNN50_RS25575 (LNN50_25580)	8017..8241	+	225	WP_000814384.1	hypothetical protein	-
LNN50_RS25580 (LNN50_25585)	8292..8552	+	261	WP_000702246.1	hypothetical protein	Toxin
LNN50_RS25585 (LNN50_25590)	8542..8793	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
LNN50_RS25590 (LNN50_25595)	9343..9567	+	225	WP_000814384.1	hypothetical protein	-
LNN50_RS25595 (LNN50_25600)	9618..9878	+	261	WP_000702246.1	hypothetical protein	-
LNN50_RS25600 (LNN50_25605)	9868..10119	+	252	WP_001132407.1	transcriptional regulator	-
LNN50_RS25605 (LNN50_25610)	10669..10947	+	279	WP_000545937.1	hypothetical protein	-
LNN50_RS25610 (LNN50_25615)	11367..11633	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
LNN50_RS25615 (LNN50_25620)	11740..12159	-	420	WP_242879860.1	hypothetical protein	-
LNN50_RS25620 (LNN50_25625)	12456..12638	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
LNN50_RS25625 (LNN50_25630)	12663..13100	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
LNN50_RS25630 (LNN50_25635)	13231..13557	-	327	WP_000206697.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..61289	61289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T223659 WP_000702246.1 NZ_CP086515:8292-8552 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T223659 NZ_CP119120:c2044546-2044439 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGTGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT223659 WP_001132407.1 NZ_CP086515:8542-8793 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT223659 NZ_CP119120:2044599-2044660 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7