Detailed information of TA system TA223648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 103240..103787
• Replicon: plasmid pEc-050-T0_1
• Accession: NZ_CP086511
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LNN50_RS23850
• Protein ID: WP_244442846.1
• Coordinates: 103509..103787 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: LNN50_RS23845
• Protein ID: WP_000079941.1
• Coordinates: 103240..103509 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN50_RS23820 (98884)	98884..99417	-	534	WP_001303319.1	transcription termination/antitermination NusG family protein	-
LNN50_RS23825 (99859)	99859..100146	-	288	WP_000074849.1	conjugal transfer protein TraA	-
LNN50_RS23830 (100064)	100064..100342	-	279	WP_219065241.1	ash family protein	-
LNN50_RS23835 (101300)	101300..102331	+	1032	WP_000907873.1	plasmid replication initiator RepA	-
LNN50_RS23840 (103027)	103027..103185	+	159	WP_000841008.1	hypothetical protein	-
LNN50_RS23845 (103240)	103240..103509	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
LNN50_RS23850 (103509)	103509..103787	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
LNN50_RS23855 (103827)	103827..104675	+	849	WP_001057996.1	3'-5' exonuclease	-
LNN50_RS23860 (104792)	104792..105265	+	474	WP_001334658.1	hypothetical protein	-
LNN50_RS23865 (105742)	105742..106008	-	267	WP_000955263.1	hypothetical protein	-
LNN50_RS23870 (106008)	106008..106502	-	495	WP_001459505.1	hypothetical protein	-
LNN50_RS23875 (106558)	106558..107160	-	603	WP_000517695.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / floR / blaTEM-30 / sul1 / qacE / ant(3'')-Ia / dfrA1	-	1..115932	115932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T223648 WP_244442846.1 NZ_CP086511:103509-103787 [Escherichia coli]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T223648 NZ_CP119109:c2616809-2616564 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT223648 WP_000079941.1 NZ_CP086511:103240-103509 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT223648 NZ_CP119109:2616230-2616595 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTACGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5