Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4346106..4346603 Replicon chromosome
Accession NZ_CP086510
Organism Escherichia coli strain Ec-050-T0

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LNN50_RS20940 Protein ID WP_001173343.1
Coordinates 4346106..4346279 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LNN50_RS20945 Protein ID WP_000398619.1
Coordinates 4346307..4346603 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN50_RS20930 (4342776) 4342776..4344233 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LNN50_RS20935 (4344470) 4344470..4345987 + 1518 WP_001295074.1 lysine--tRNA ligase -
LNN50_RS20940 (4346106) 4346106..4346279 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LNN50_RS20945 (4346307) 4346307..4346603 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LNN50_RS20950 (4346830) 4346830..4347102 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LNN50_RS20955 (4347114) 4347114..4347344 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LNN50_RS20960 (4347525) 4347525..4349156 + 1632 WP_001216477.1 sensor histidine kinase -
LNN50_RS20965 (4349153) 4349153..4349872 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T223643 WP_001173343.1 NZ_CP086510:c4346279-4346106 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T223643 NZ_CP119109:767291-767500 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT223643 WP_000398619.1 NZ_CP086510:c4346603-4346307 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT223643 NZ_CP119109:766986-767279 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References