Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4243760..4244355 Replicon chromosome
Accession NZ_CP086510
Organism Escherichia coli strain Ec-050-T0

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag LNN50_RS20405 Protein ID WP_000239579.1
Coordinates 4243760..4244110 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag LNN50_RS20410 Protein ID WP_001223208.1
Coordinates 4244104..4244355 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN50_RS20385 (4239206) 4239206..4240228 - 1023 WP_001295197.1 ABC transporter permease -
LNN50_RS20390 (4240242) 4240242..4241744 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
LNN50_RS20395 (4241884) 4241884..4242840 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
LNN50_RS20400 (4243150) 4243150..4243680 + 531 WP_000055072.1 inorganic diphosphatase -
LNN50_RS20405 (4243760) 4243760..4244110 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
LNN50_RS20410 (4244104) 4244104..4244355 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
LNN50_RS20415 (4244567) 4244567..4244908 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
LNN50_RS20420 (4244911) 4244911..4248690 - 3780 WP_000060978.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T223642 WP_000239579.1 NZ_CP086510:c4244110-4243760 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T223642 NZ_CP119109:708413-708517 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT223642 WP_001223208.1 NZ_CP086510:c4244355-4244104 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT223642 NZ_CP119109:c708676-708512 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References