Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4127438..4128014 Replicon chromosome
Accession NZ_CP086510
Organism Escherichia coli strain Ec-050-T0

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LNN50_RS19800 Protein ID WP_001297643.1
Coordinates 4127727..4128014 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LNN50_RS19795 Protein ID WP_000063160.1
Coordinates 4127438..4127740 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN50_RS19780 (4124034) 4124034..4126184 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LNN50_RS19785 (4126234) 4126234..4126437 + 204 WP_000467859.1 YbdD/YjiX family protein -
LNN50_RS19790 (4126448) 4126448..4127404 + 957 WP_001297640.1 GTPase -
LNN50_RS19795 (4127438) 4127438..4127740 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LNN50_RS19800 (4127727) 4127727..4128014 - 288 WP_001297643.1 BrnT family toxin Toxin
LNN50_RS26360 (4128213) 4128213..4128909 - 697 Protein_3900 IS1 family transposase -
LNN50_RS19815 (4129224) 4129224..4130456 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
LNN50_RS19820 (4130497) 4130497..4131777 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4128213..4128422 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T223641 WP_001297643.1 NZ_CP086510:c4128014-4127727 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T223641 NZ_CP119109:411628-411735 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT223641 WP_000063160.1 NZ_CP086510:c4127740-4127438 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT223641 NZ_CP119109:c411812-411752 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References