Detailed information of TA system TA223637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2541566..2542204
• Replicon: chromosome
• Accession: NZ_CP086510
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: LNN50_RS12170
• Protein ID: WP_001447010.1
• Coordinates: 2542028..2542204 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LNN50_RS12165
• Protein ID: WP_001270286.1
• Coordinates: 2541566..2541982 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN50_RS12145 (2536718)	2536718..2537659	-	942	WP_001251304.1	ABC transporter permease	-
LNN50_RS12150 (2537660)	2537660..2538673	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LNN50_RS12155 (2538691)	2538691..2539836	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LNN50_RS12160 (2540081)	2540081..2541487	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LNN50_RS12165 (2541566)	2541566..2541982	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LNN50_RS12170 (2542028)	2542028..2542204	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LNN50_RS12175 (2542426)	2542426..2542656	+	231	WP_000494244.1	YncJ family protein	-
LNN50_RS12180 (2542748)	2542748..2544709	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LNN50_RS12185 (2544782)	2544782..2545318	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LNN50_RS12190 (2545410)	2545410..2546585	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T223637 WP_001447010.1 NZ_CP086510:c2542204-2542028 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T223637 NZ_CP119108:c3118017-3117694 [Microbacterium sp. KACC 23027]
GTGTGGGTCGACTTCGGCGAGCCGAGAGGGTCGGAGCCAGGAAAGGTCCGCCCGGCGGTGATCCTTCAGGATGATTGGCT
GCTCGCCTCGTCGATCGCCACTGTCGTCGTCGTCCCGCTCACCTCGAACACGCGGCTGGCTGCTTTCCCGGGCAACGTGC
TCATCCCATCTGAGGCATCAGGACTCGAGAAGGACTCGGTTGCTGTGGTCTCGCAGATCGGTCCGGTCAGTCGCGAGTAC
ATAGTTCCGTATCCTGTCGGGGCCCTCGCCAACTACTTGTTGAGCGATGTCGCGGCCGGAGTACGGCTCGTCATCGGACT
CTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT223637 WP_001270286.1 NZ_CP086510:c2541982-2541566 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT223637 NZ_CP119108:c3118274-3118035 [Microbacterium sp. KACC 23027]
ATGAAGACAGCCATTTCGGTTCCCGACGGCGACTTCGAACGATTCGAGCGCACTGCGCGCAAGCACGGCATGAATCGGTC
GGAGTTCTATCGGCTCGCCGCGCAGAAGCTCGCGGACGAACTCGAGGGGGAGTCCGAACTCACGGCGATCGCGAATGACG
TGATCGAACGTGTCGGTCAGCCCGCCGACGCCGAGTTCCTCCGCGAGTCACATCGGGCCATCCGGGGCACCGAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8