Detailed information of TA system TA223627

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1920512..1921347
• Replicon: chromosome
• Accession: NZ_CP086510
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LNN50_RS09200
• Protein ID: WP_042046912.1
• Coordinates: 1920512..1920889 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LNN50_RS09205
• Protein ID: WP_135145692.1
• Coordinates: 1920979..1921347 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN50_RS09165 (1916032)	1916032..1916505	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
LNN50_RS09170 (1916703)	1916703..1917761	+	1059	WP_001200895.1	FUSC family protein	-
LNN50_RS09175 (1917933)	1917933..1918262	+	330	WP_000450409.1	DUF496 family protein	-
LNN50_RS09180 (1918363)	1918363..1918728	-	366	WP_001280455.1	EutP/PduV family microcompartment system protein	-
LNN50_RS09185 (1918999)	1918999..1919388	-	390	WP_077875581.1	transposase	-
LNN50_RS09190 (1920186)	1920186..1920266	-	81	Protein_1811	hypothetical protein	-
LNN50_RS09195 (1920366)	1920366..1920515	-	150	Protein_1812	DUF5983 family protein	-
LNN50_RS09200 (1920512)	1920512..1920889	-	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
LNN50_RS09205 (1920979)	1920979..1921347	-	369	WP_135145692.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LNN50_RS09210 (1921510)	1921510..1921731	-	222	WP_000692345.1	DUF987 domain-containing protein	-
LNN50_RS09215 (1921794)	1921794..1921874	-	81	Protein_1816	JAB domain-containing protein	-
LNN50_RS09220 (1921961)	1921961..1922707	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
LNN50_RS09225 (1922722)	1922722..1924262	-	1541	Protein_1818	IS21-like element ISEc12 family transposase	-
LNN50_RS09230 (1924454)	1924454..1924855	-	402	Protein_1819	JAB domain-containing protein	-
LNN50_RS09235 (1924871)	1924871..1925344	-	474	WP_000855059.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T223627 WP_042046912.1 NZ_CP086510:c1920889-1920512 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T223627 NZ_CP119076:c4343636-4343319 [Klebsiella aerogenes]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGGAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTTGAGCAGCACTATGGCCTGGCACTGAACGACACGCCATTTAGTGATGAAACTGTGATTCAGGAACACATTG
ATGCCGGTATCACGCTGTCTGATGCCATTAACTTTCTGGTGGATAAGTACGAGCTGGTTCGTATCGATCGCAGAGGGTTT
AGTTGGCAAGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCACGACAGGCAACTGGCTTGTTACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13545.30 Da
• Isoelectric Point: 6.4764

>AT223627 WP_135145692.1 NZ_CP086510:c1921347-1920979 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPHHQHTVTLYAKGLTCEADTLGSCGYAYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT223627 NZ_CP119076:c4343977-4343657 [Klebsiella aerogenes]
ATGAGAAGTCATGTTACATGGGGCCTGCAGCGGGATATCACGCCACGCCTGGGAGCTCGTCTGGTGCAGGAGGGCAACCG
ACTGCACTACCTGACTGACCGGGCCAGCATTACCGGTCAGTTCAGTGATGCCGAATGTCTTAAGCTGGATGTGGCATTTC
CGCATTTTATCAGCCAGATGGAATCCATGCTGACCACTAGTGAAATGAATCCCCGCCATGCCCACTGTGTCACCCTGTAC
CACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAACGCTA
A