Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933025..933679 Replicon chromosome
Accession NZ_CP086510
Organism Escherichia coli strain Ec-050-T0

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LNN50_RS04580 Protein ID WP_000244777.1
Coordinates 933272..933679 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LNN50_RS04575 Protein ID WP_000354046.1
Coordinates 933025..933291 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN50_RS04550 (928194) 928194..928937 + 744 WP_000951964.1 SDR family oxidoreductase -
LNN50_RS04555 (928994) 928994..930427 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LNN50_RS04560 (930472) 930472..930783 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LNN50_RS04565 (930947) 930947..931606 + 660 WP_000250274.1 hemolysin III family protein -
LNN50_RS04570 (931802) 931802..932782 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LNN50_RS04575 (933025) 933025..933291 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LNN50_RS04580 (933272) 933272..933679 + 408 WP_000244777.1 protein YgfX Toxin
LNN50_RS04585 (933719) 933719..934240 - 522 WP_001055874.1 flavodoxin FldB -
LNN50_RS04590 (934352) 934352..935248 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LNN50_RS04595 (935273) 935273..935983 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LNN50_RS04600 (935989) 935989..937722 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T223624 WP_000244777.1 NZ_CP086510:933272-933679 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223624 NZ_CP119076:1897744-1897847 [Klebsiella aerogenes]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223624 WP_000354046.1 NZ_CP086510:933025-933291 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223624 NZ_CP119076:c1897855-1897693 [Klebsiella aerogenes]
TAATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTGACCGTGACAGGTTTTCCAGTCCGCGACAAAAGT
GGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References