Detailed information of TA system TA223614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 41249..41850
• Replicon: plasmid pEc-050-T5-MAC_3
• Accession: NZ_CP086503
• Organism: Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LNN49_RS24860
• Protein ID: WP_168420618.1
• Coordinates: 41470..41850 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: LNN49_RS24855
• Protein ID: WP_001190712.1
• Coordinates: 41249..41470 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN49_RS24795 (LNN49_24800)	36671..36865	+	195	WP_000224731.1	hypothetical protein	-
LNN49_RS24800 (LNN49_24805)	36867..37076	+	210	WP_000951710.1	hypothetical protein	-
LNN49_RS24805 (LNN49_24810)	37073..37198	+	126	Protein_55	DUF551 domain-containing protein	-
LNN49_RS24810 (LNN49_24815)	37709..37900	+	192	WP_242806062.1	DUF551 domain-containing protein	-
LNN49_RS24815 (LNN49_24820)	37893..38174	+	282	WP_102218741.1	ASCH domain-containing protein	-
LNN49_RS24820 (LNN49_24825)	38167..38571	+	405	WP_080077662.1	hypothetical protein	-
LNN49_RS24825 (LNN49_24830)	38584..38877	+	294	WP_075660654.1	hypothetical protein	-
LNN49_RS24830 (LNN49_24835)	38877..39053	+	177	WP_157902266.1	hypothetical protein	-
LNN49_RS24835 (LNN49_24840)	39133..39342	+	210	WP_075660655.1	hypothetical protein	-
LNN49_RS24840 (LNN49_24845)	39339..40097	+	759	WP_072756859.1	hypothetical protein	-
LNN49_RS24845 (LNN49_24850)	40412..40663	+	252	WP_063074807.1	DNA polymerase III subunit theta	-
LNN49_RS24850 (LNN49_24855)	40787..41176	+	390	WP_168420616.1	S24 family peptidase	-
LNN49_RS24855 (LNN49_24860)	41249..41470	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LNN49_RS24860 (LNN49_24865)	41470..41850	+	381	WP_168420618.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LNN49_RS24865 (LNN49_24870)	41934..42734	-	801	WP_168420620.1	hypothetical protein	-
LNN49_RS24870 (LNN49_24875)	42741..43403	-	663	WP_168420622.1	DUF2829 domain-containing protein	-
LNN49_RS24875 (LNN49_24880)	43418..43912	-	495	WP_000640906.1	dUTP diphosphatase	-
LNN49_RS24880 (LNN49_24885)	43909..44385	-	477	WP_000861174.1	hypothetical protein	-
LNN49_RS24885 (LNN49_24890)	44382..44726	-	345	WP_001191776.1	hypothetical protein	-
LNN49_RS24890 (LNN49_24895)	44800..45828	-	1029	WP_168420624.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90296	90296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13605.50 Da
• Isoelectric Point: 5.6406

>T223614 WP_168420618.1 NZ_CP086503:41470-41850 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T223614 NZ_CP119014:c36024-35875 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT223614 WP_001190712.1 NZ_CP086503:41249-41470 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT223614 NZ_CP119014:36069-36128 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6