Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4127444..4128020 Replicon chromosome
Accession NZ_CP086500
Organism Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LNN49_RS19800 Protein ID WP_001297643.1
Coordinates 4127733..4128020 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LNN49_RS19795 Protein ID WP_000063160.1
Coordinates 4127444..4127746 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN49_RS19780 (4124040) 4124040..4126190 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LNN49_RS19785 (4126240) 4126240..4126443 + 204 WP_000467859.1 YbdD/YjiX family protein -
LNN49_RS19790 (4126454) 4126454..4127410 + 957 WP_001297640.1 GTPase -
LNN49_RS19795 (4127444) 4127444..4127746 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LNN49_RS19800 (4127733) 4127733..4128020 - 288 WP_001297643.1 BrnT family toxin Toxin
LNN49_RS26375 (4128219) 4128219..4128915 - 697 Protein_3898 IS1 family transposase -
LNN49_RS19815 (4129230) 4129230..4130462 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
LNN49_RS19820 (4130503) 4130503..4131783 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4128219..4128428 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T223599 WP_001297643.1 NZ_CP086500:c4128020-4127733 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T223599 NZ_CP118992:52966-53316 [Aeromonas allosaccharophila]
ATGTGGACAGTAAAAACGACCTCTCTATTCGATGTCTGGTTTGATGAACAAGACGAGGCCACGCAGGAGAAGGTTTTGGC
TGGGTTGCTTGCCTTGGCACAGGGCGGTCCATCAGTAGGGCGACCCTTAGTTGATACCATCAAGGGGTCTCGCTTTACGA
ACCTCAAAGAGCTGAGAGTTCAGCACCAGGGGGATCCGCTACGAGCGTTCTTTGCTTTTGACCCGTTGCGGCAAGCTATC
GTGTTGTGTGCTGGAAATAAGGGCGGTAACGATAAGCGGTTTTACAAAGAGATGTTGCCGGTCGCTGAGGCGGAATACGC
CAAGCACTTAACTGAGTTGGAGGTAGGGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT223599 WP_000063160.1 NZ_CP086500:c4127746-4127444 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT223599 NZ_CP118992:53316-53618 [Aeromonas allosaccharophila]
ATGGGAAGAACACTGAATGAGATGCTGGCCTCCCGCTCGGTGGATAGTCAGCAGCGGATCCAGGAGATGGCTAACGAGCT
GCTGCTGGAGGTACGCTTGCAAGCGCTGCGTGAAGAGTTGGAAGTGTCCCAGGTGGAGTTGGCCTCTGCCCTCGGGATCA
GCCAACCGGCCGTTGTGGCGATGGAGCAGCGTGGGAATGATATCAAGCTATCCACCATGAAGCGTTACGTGGAAGCAATG
GGTGGGAAGCTGCGTGTTGATGTTGAGCTTCCTACAGGGAGGCATGTCGGTTTCAACGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References