Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3573593..3574211 Replicon chromosome
Accession NZ_CP086500
Organism Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LNN49_RS17170 Protein ID WP_001291435.1
Coordinates 3573993..3574211 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LNN49_RS17165 Protein ID WP_000344800.1
Coordinates 3573593..3573967 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN49_RS17155 (3568682) 3568682..3569875 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN49_RS17160 (3569898) 3569898..3573047 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LNN49_RS17165 (3573593) 3573593..3573967 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LNN49_RS17170 (3573993) 3573993..3574211 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LNN49_RS17175 (3574383) 3574383..3574934 + 552 WP_000102564.1 maltose O-acetyltransferase -
LNN49_RS17180 (3575050) 3575050..3575520 + 471 WP_000136192.1 YlaC family protein -
LNN49_RS17185 (3575684) 3575684..3577234 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LNN49_RS17190 (3577276) 3577276..3577629 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LNN49_RS17200 (3578008) 3578008..3578319 + 312 WP_000409911.1 MGMT family protein -
LNN49_RS17205 (3578350) 3578350..3578922 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223596 WP_001291435.1 NZ_CP086500:3573993-3574211 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223596 NZ_CP118990:c178804-178517 [Aeromonas allosaccharophila]
ATGACCTTTGAACTCGAGTTTGATCCGCGAGCGTGGAAAGAGTGGAAAAAGTTGGGCGATACCGTTCGCCAGCAGTTCAA
AAAGAAACTTTCAGAGGTGATCGAGCGCCCCAGGATTGAGGCAAACAGGCTGAATGGGATGTCTGATTGCTACAAGATCA
AATTGCGTGGCGCGGGGTATCGGTTGGTTTACCAGGTACAGGATGATCGGGTGGTGGTATTCGTGGTCGCAGTGGGGAGG
CGCGAACGGGAAGAGGTATATCTGGATGCCATGTCACGTCTTGATTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223596 WP_000344800.1 NZ_CP086500:3573593-3573967 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223596 NZ_CP118990:c179036-178794 [Aeromonas allosaccharophila]
ATGGGCACGATTAACCTGCGCATTGATGATGAACTGAAGGCACGTTCATTTGCGGTGCTGGACAAATTGGGGGTGACCCC
CTCGGATATTATCCGGCAAACCCTGGAGTATGTGGCCTCTCAGGGGCGGAGCCCGTTTCGCACTACCGTGGTGACGGAGG
ACGAAGAGGCACTGTTAGCCATCGTGCGAGAGCGCTTGGCCAGCCCGCAGAAAGGCATCAAGGTATCGCTGGATGACCTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References