Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933026..933680 Replicon chromosome
Accession NZ_CP086500
Organism Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LNN49_RS04580 Protein ID WP_000244777.1
Coordinates 933273..933680 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LNN49_RS04575 Protein ID WP_000354046.1
Coordinates 933026..933292 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN49_RS04550 (928195) 928195..928938 + 744 WP_000951964.1 SDR family oxidoreductase -
LNN49_RS04555 (928995) 928995..930428 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LNN49_RS04560 (930473) 930473..930784 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LNN49_RS04565 (930948) 930948..931607 + 660 WP_000250274.1 hemolysin III family protein -
LNN49_RS04570 (931803) 931803..932783 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LNN49_RS04575 (933026) 933026..933292 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LNN49_RS04580 (933273) 933273..933680 + 408 WP_000244777.1 protein YgfX Toxin
LNN49_RS04585 (933720) 933720..934241 - 522 WP_001055874.1 flavodoxin FldB -
LNN49_RS04590 (934353) 934353..935249 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LNN49_RS04595 (935274) 935274..935984 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LNN49_RS04600 (935990) 935990..937723 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T223582 WP_000244777.1 NZ_CP086500:933273-933680 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223582 NZ_CP118974:745648-746019 [Mammaliicoccus vitulinus]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCACCAGTCACTGGTTCTGAACAAGGGGGGACAAGACCAGTCGTAAT
TATCCAAAATGATACGGGTAACAGATATAGTCCGACTGTTATTGTAGCAGCAATTACTGGTAAGATAAATAAAGCTAAAA
TACCTACACACGTAGAAATTGAAGCAGCGAAATATAAACTAGATAGAGATTCAGTTATTTTGCTAGAACAAATTAGAACC
ATTGATAAGAAACGTTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATACAGCTATAGCAAT
CAGCCTCAACCTAACTTTGCAACAAAAATTTGATGCATTAGGTAATACATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223582 WP_000354046.1 NZ_CP086500:933026-933292 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223582 NZ_CP118974:745480-745647 [Mammaliicoccus vitulinus]
ATGACATCTTTAACACAAAATCGTACTCAAAGTATAGAACAAACACTGAAAGAAGGATATCGTTCTATGGCTGATTTAAA
TCTCTCCCTTGCATCTGAAGCATACTATAGTGAATGTGAAGCTTGTGATTCTAATGAATCGCACTTAGCATCGCAACAAG
GTGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References