Detailed information of TA system TA022358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3743533..3744117
• Replicon: chromosome
• Accession: NC_009767
• Organism: Roseiflexus castenholzii DSM 13941

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: RCAS_RS24065
• Protein ID: WP_012121370.1
• Coordinates: 3743533..3743697 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A7NN15
• Locus tag: RCAS_RS14885
• Protein ID: WP_012121371.1
• Coordinates: 3743704..3744117 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RCAS_RS14865	3738591..3740126	+	1536	WP_198136051.1	acyl-CoA carboxylase subunit beta	-
RCAS_RS14870	3740137..3741618	+	1482	WP_012121368.1	ATP-grasp domain-containing protein	-
RCAS_RS14875	3741630..3742124	+	495	WP_012121369.1	acetyl-CoA carboxylase biotin carboxyl carrier protein subunit	-
RCAS_RS24065	3743533..3743697	+	165	WP_012121370.1	type II toxin-antitoxin system HicA family toxin	Toxin
RCAS_RS14885	3743704..3744117	+	414	WP_012121371.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RCAS_RS14890	3744098..3744670	+	573	WP_012121372.1	Uma2 family endonuclease	-
RCAS_RS14895	3744677..3745711	-	1035	WP_157042660.1	sigma-70 family RNA polymerase sigma factor	-
RCAS_RS14900	3745802..3747088	-	1287	WP_157042661.1	DUF58 domain-containing protein	-
RCAS_RS14905	3747091..3748485	-	1395	WP_012121375.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-48)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-124)

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6373.45 Da
• Isoelectric Point: 10.6309

>T22358 WP_012121370.1 NC_009767:3743533-3743697 [Roseiflexus castenholzii DSM 13941]
MCMWMLVHVRGSHHHYKHPNKPGRVTIPHPKKDLPVGTLKSIYRQAGWSWSEKE

Nucleotide

• Length: 165 bp

>T22358 NC_009767:3743533-3743697 [Roseiflexus castenholzii DSM 13941]
ATGTGTATGTGGATGCTGGTCCACGTTCGGGGCAGCCACCACCACTACAAACATCCCAATAAGCCTGGACGCGTGACTAT
TCCGCATCCAAAGAAAGATTTACCTGTAGGAACTTTGAAGAGTATCTACCGTCAAGCCGGTTGGTCCTGGTCAGAGAAGG
AGTGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15024.97 Da
• Isoelectric Point: 4.6685

>AT22358 WP_012121371.1 NC_009767:3743704-3744117 [Roseiflexus castenholzii DSM 13941]
MHYPVVIHKDTESDYGVTVPDLPGCFSAGETLDEALQEAIEAIECHLEGLLADGEPIPTPKPVELHQSNPDYAGGIWAFV
TVDVTKLSGKTRRINITLPERVLHLVNKYASERGESRSGLITQAVLEYIGSRETLAR

Nucleotide

• Length: 414 bp

>AT22358 NC_009767:3743704-3744117 [Roseiflexus castenholzii DSM 13941]
ATGCATTACCCTGTTGTCATCCATAAGGATACTGAAAGCGATTATGGCGTTACTGTGCCTGACTTGCCTGGTTGCTTCAG
TGCGGGTGAAACACTCGATGAAGCATTGCAAGAAGCCATCGAAGCCATTGAGTGCCATCTAGAAGGCTTATTAGCAGATG
GCGAGCCTATCCCAACGCCAAAACCGGTTGAATTGCATCAAAGTAATCCCGATTATGCCGGAGGGATCTGGGCATTCGTT
ACTGTTGATGTTACAAAGCTATCAGGTAAAACGCGCAGGATCAATATTACGTTGCCTGAGCGTGTATTACATCTCGTGAA
TAAATATGCGAGTGAACGTGGTGAGAGTCGTTCAGGGTTGATAACCCAGGCGGTGCTTGAATATATTGGGTCCCGTGAAA
CGCTCGCTCGTTAA

Structures

Antitoxin

• AlphaFold DB: A7NN15