Detailed information of TA system TA022355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1228256..1229173
• Replicon: chromosome
• Accession: NC_009725
• Organism: Bacillus amyloliquefaciens subsp. plantarum str. FZB42

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: RBAM_RS06415
• Protein ID: WP_012117368.1
• Coordinates: 1228427..1229173 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: RBAM_RS06410
• Protein ID: WP_003154807.1
• Coordinates: 1228256..1228426 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBAM_RS06365	1223390..1223902	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
RBAM_RS06370	1224015..1224236	+	222	Protein_1195	terminase	-
RBAM_RS06375	1224645..1225112	+	468	WP_077411063.1	hypothetical protein	-
RBAM_RS06380	1225125..1225496	+	372	WP_012117365.1	hypothetical protein	-
RBAM_RS06385	1225501..1225698	+	198	WP_012117366.1	XkdX family protein	-
RBAM_RS06390	1225755..1226516	+	762	WP_012117367.1	hypothetical protein	-
RBAM_RS06395	1226568..1226831	+	264	WP_003154815.1	hemolysin XhlA family protein	-
RBAM_RS06400	1226845..1227108	+	264	WP_003154813.1	phage holin	-
RBAM_RS06405	1227122..1228000	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
RBAM_RS06410	1228256..1228426	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
RBAM_RS06415	1228427..1229173	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
RBAM_RS06420	1229278..1230276	-	999	WP_007407255.1	inorganic phosphate transporter	-
RBAM_RS06425	1230289..1230906	-	618	WP_003154804.1	DUF47 domain-containing protein	-
RBAM_RS06430	1231192..1232508	-	1317	WP_007610842.1	amino acid permease	-
RBAM_RS06435	1232831..1233781	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T22355 WP_012117368.1 NC_009725:c1229173-1228427 [Bacillus velezensis FZB42]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T22355 NC_009725:c1229173-1228427 [Bacillus velezensis FZB42]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGAATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAAGCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT22355 WP_003154807.1 NC_009725:c1228426-1228256 [Bacillus velezensis FZB42]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT22355 NC_009725:c1228426-1228256 [Bacillus velezensis FZB42]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14