Detailed information of TA system TA022354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 493011..493648
• Replicon: chromosome
• Accession: NC_009725
• Organism: Bacillus amyloliquefaciens subsp. plantarum str. FZB42

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: RBAM_RS02510
• Protein ID: WP_003156187.1
• Coordinates: 493298..493648 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: RBAM_RS02505
• Protein ID: WP_003156188.1
• Coordinates: 493011..493292 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBAM_RS02485	489376..489975	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
RBAM_RS02490	490068..490433	+	366	WP_012116869.1	holo-ACP synthase	-
RBAM_RS02495	490598..491605	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
RBAM_RS02500	491722..492891	+	1170	WP_012116870.1	alanine racemase	-
RBAM_RS02505	493011..493292	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
RBAM_RS02510	493298..493648	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
RBAM_RS02515	493766..494587	+	822	WP_003156182.1	STAS domain-containing protein	-
RBAM_RS02520	494592..494957	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
RBAM_RS02525	494960..495361	+	402	WP_003156178.1	anti-sigma regulatory factor	-
RBAM_RS02530	495373..496380	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
RBAM_RS02535	496444..496773	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
RBAM_RS02540	496770..497252	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
RBAM_RS02545	497218..498006	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
RBAM_RS02550	498006..498608	+	603	WP_012116871.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T22354 WP_003156187.1 NC_009725:493298-493648 [Bacillus velezensis FZB42]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T22354 NC_009725:493298-493648 [Bacillus velezensis FZB42]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT22354 WP_003156188.1 NC_009725:493011-493292 [Bacillus velezensis FZB42]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT22354 NC_009725:493011-493292 [Bacillus velezensis FZB42]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5