223536

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 29041..29506 Replicon plasmid pEc-050-T20-MAC_6
Accession NZ_CP086486
Organism Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)


Gene name TsxA Uniprot ID A0A5Y6UAV0
Locus tag LNN46_RS26090 Protein ID WP_001230708.1
Coordinates 29041..29292 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag LNN46_RS26095 Protein ID WP_000801440.1
Coordinates 29279..29506 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN46_RS26070 (LNN46_26075) 26561..26896 + 336 WP_001147119.1 type II toxin-antitoxin system RelE/ParE family toxin -
LNN46_RS26075 (LNN46_26080) 26893..27243 + 351 WP_000127483.1 hypothetical protein -
LNN46_RS26080 (LNN46_26085) 27882..28397 + 516 WP_001025396.1 J domain-containing protein -
LNN46_RS26085 (LNN46_26090) 28502..29044 + 543 WP_001083908.1 hypothetical protein -
LNN46_RS26090 (LNN46_26095) 29041..29292 + 252 WP_001230708.1 hypothetical protein Toxin
LNN46_RS26095 (LNN46_26100) 29279..29506 + 228 WP_000801440.1 hypothetical protein Antitoxin
LNN46_RS26100 (LNN46_26105) 29898..30197 + 300 WP_000650313.1 hypothetical protein -
LNN46_RS26105 (LNN46_26110) 30289..30654 + 366 WP_000854262.1 hypothetical protein -
LNN46_RS26110 (LNN46_26115) 30929..31102 + 174 WP_071821486.1 type II toxin-antitoxin system HicB family antitoxin -
LNN46_RS26115 (LNN46_26120) 31465..31968 + 504 WP_000781818.1 DNA distortion polypeptide 1 -
LNN46_RS26120 (LNN46_26125) 31972..33201 + 1230 WP_000539545.1 DNA relaxase/nickase TaxC -
LNN46_RS26125 (LNN46_26130) 33278..33571 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
LNN46_RS26130 (LNN46_26135) 33568..33783 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33826 33826


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-94)

Antitoxin

Ribbon-helix-helix protein, CopG

(8-45)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9750.19 Da        Isoelectric Point: 9.8104

>T223536 WP_001230708.1 NZ_CP086486:29041-29292 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T223536 NZ_CP118940:c131263-130970 [Aeromonas caviae]
ATGCCACCAGTGATCTTTGCCCCTGCCGCCCTGCGAGACCTGCAACGCCTACGTGAATTCCTGCGCCCTAAAAGCCCGCA
GGTCGCACAGCGGGCCGCAACAACAATCCAGCTGTCCCTACGCCAGCTCGCAGCCCAACCCTCAATGGGCCGCCCCATAG
AAGGACTGCCAGAGGCGTTTCGTGAGTGGGTGATCCCCTTTGGTGACAGCGGCTATTTAGCCCGCTATCGCATTGAACCG
GATGTCATCGTGGTGCTCGCTGTCCGCCATCAACGCGAAGTTGGCTACTCATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT223536 WP_000801440.1 NZ_CP086486:29279-29506 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT223536 NZ_CP118940:c131538-131251 [Aeromonas caviae]
ATGCCCACACAATCCAAACCGGTCTCGATCAAACTCGACGCCGATACCCAGGAACGCATCAAACGCCTGGCAACCTCCCG
CGAACGCTCAGCCCATTGGCTCATGCGCGAAGCCATCAGCCAGTATGTCGAGCGCGAAGAACTGCGCGAGACCTTCCACC
GCGACACTCTGGAGGCCTGGCAGGAGTTCCAGGAGACCGGCCAGTATGCCACCGCTCAAGATGTGGATCAGTGGCTAGCC
TCTTGGGGCACTGATGCCGAAGGAGCGCCTCCTGCATGCCACCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Y6UAV0

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References