Detailed information of TA system TA223531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 41197..41798
• Replicon: plasmid pEc-050-T20-MAC_3
• Accession: NZ_CP086483
• Organism: Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LNN46_RS24860
• Protein ID: WP_168420618.1
• Coordinates: 41418..41798 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: LNN46_RS24855
• Protein ID: WP_001190712.1
• Coordinates: 41197..41418 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN46_RS24795 (LNN46_24800)	36619..36813	+	195	WP_000224731.1	hypothetical protein	-
LNN46_RS24800 (LNN46_24805)	36815..37024	+	210	WP_000951710.1	hypothetical protein	-
LNN46_RS24805 (LNN46_24810)	37021..37146	+	126	Protein_54	DUF551 domain-containing protein	-
LNN46_RS24810 (LNN46_24815)	37657..37848	+	192	WP_242806062.1	DUF551 domain-containing protein	-
LNN46_RS24815 (LNN46_24820)	37841..38122	+	282	WP_102218741.1	ASCH domain-containing protein	-
LNN46_RS24820 (LNN46_24825)	38115..38519	+	405	WP_080077662.1	hypothetical protein	-
LNN46_RS24825 (LNN46_24830)	38532..38825	+	294	WP_075660654.1	hypothetical protein	-
LNN46_RS24830 (LNN46_24835)	38825..39001	+	177	WP_157902266.1	hypothetical protein	-
LNN46_RS24835 (LNN46_24840)	39081..39290	+	210	WP_075660655.1	hypothetical protein	-
LNN46_RS24840 (LNN46_24845)	39287..40045	+	759	WP_072756859.1	hypothetical protein	-
LNN46_RS24845 (LNN46_24850)	40360..40611	+	252	WP_063074807.1	DNA polymerase III subunit theta	-
LNN46_RS24850 (LNN46_24855)	40735..41124	+	390	WP_168420616.1	S24 family peptidase	-
LNN46_RS24855 (LNN46_24860)	41197..41418	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LNN46_RS24860 (LNN46_24865)	41418..41798	+	381	WP_168420618.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LNN46_RS24865 (LNN46_24870)	41882..42682	-	801	WP_168420620.1	hypothetical protein	-
LNN46_RS24870 (LNN46_24875)	42689..43351	-	663	WP_168420622.1	DUF2829 domain-containing protein	-
LNN46_RS24875 (LNN46_24880)	43366..43860	-	495	WP_000640906.1	dUTP diphosphatase	-
LNN46_RS24880 (LNN46_24885)	43857..44333	-	477	WP_000861174.1	hypothetical protein	-
LNN46_RS24885 (LNN46_24890)	44330..44674	-	345	WP_001191776.1	hypothetical protein	-
LNN46_RS24890 (LNN46_24895)	44748..45776	-	1029	WP_168420624.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89996	89996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13605.50 Da
• Isoelectric Point: 5.6406

>T223531 WP_168420618.1 NZ_CP086483:41418-41798 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T223531 NZ_CP118937:44815-45297 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT223531 WP_001190712.1 NZ_CP086483:41197-41418 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT223531 NZ_CP118937:44561-44827 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6