Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 76976..77619 Replicon plasmid pEc-050-T20-MAC_2
Accession NZ_CP086482
Organism Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LNN46_RS24430 Protein ID WP_001034044.1
Coordinates 77203..77619 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LNN46_RS24425 Protein ID WP_001261286.1
Coordinates 76976..77206 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN46_RS24405 (73607) 73607..74362 - 756 WP_000852146.1 replication initiation protein RepE -
LNN46_RS24410 (75084) 75084..75890 - 807 WP_000016970.1 site-specific integrase -
LNN46_RS24415 (75891) 75891..76196 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
LNN46_RS24420 (76198) 76198..76416 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
LNN46_RS24425 (76976) 76976..77206 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LNN46_RS24430 (77203) 77203..77619 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN46_RS24435 (77694) 77694..79259 + 1566 WP_001128474.1 AAA family ATPase -
LNN46_RS24440 (79244) 79244..80266 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aac(3)-IIa / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..99476 99476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T223529 WP_001034044.1 NZ_CP086482:77203-77619 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T223529 NZ_CP118936:c4650256-4649972 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT223529 WP_001261286.1 NZ_CP086482:76976-77206 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT223529 NZ_CP118936:c4650488-4650246 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References