Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4346118..4346615 Replicon chromosome
Accession NZ_CP086480
Organism Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LNN46_RS20945 Protein ID WP_001173343.1
Coordinates 4346118..4346291 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LNN46_RS20950 Protein ID WP_000398619.1
Coordinates 4346319..4346615 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN46_RS20935 (4342788) 4342788..4344245 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LNN46_RS20940 (4344482) 4344482..4345999 + 1518 WP_001295074.1 lysine--tRNA ligase -
LNN46_RS20945 (4346118) 4346118..4346291 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LNN46_RS20950 (4346319) 4346319..4346615 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LNN46_RS20955 (4346842) 4346842..4347114 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LNN46_RS20960 (4347126) 4347126..4347356 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LNN46_RS20965 (4347537) 4347537..4349168 + 1632 WP_001216477.1 sensor histidine kinase -
LNN46_RS20970 (4349165) 4349165..4349884 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T223518 WP_001173343.1 NZ_CP086480:c4346291-4346118 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T223518 NZ_CP118932:c4250356-4250081 [Serratia marcescens]
ATGTTGATCGTCAAATGGACTGACGAAGCAAAAACGGGTTTATACACGCTGCTTGCCTTTATCGCGGAGCGAAATCCCGT
TGCCGCTGAGGCTCTGTTGCAACGCATTGAAGAGTCGGTTATCCCGTTAGCAGAACATCCTTATCTGTTCCGGCCAGGCA
GGGTAAACGGGACGCGTGAAATGGTCGTTCATCCTAATTACATTGTGGTTTACCGCGTGCTGGCTGCACATATTGAAATA
GTGGGTGTCCTGCATGCTCGTCAGGAATACCCTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT223518 WP_000398619.1 NZ_CP086480:c4346615-4346319 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT223518 NZ_CP118932:c4250553-4250362 [Serratia marcescens]
ATGCCGACAAAATTGGATCCCATCGTTTCTGAGTTTGAGACTCAAGAGCAGGCTGATAGCTATGATCGTTGGTTCAGAAG
CACGGTGGAATCTTCCATCAATAGCGACACCCAGCGCGTCCCCCATGACGAGGTCATGGCCGGTGCTCGCCACATTATCG
AACAGGCAAAAGCCAAAAGAAAATCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References