Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4127445..4128021 Replicon chromosome
Accession NZ_CP086480
Organism Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LNN46_RS19805 Protein ID WP_001297643.1
Coordinates 4127734..4128021 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LNN46_RS19800 Protein ID WP_000063160.1
Coordinates 4127445..4127747 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN46_RS19785 (4124041) 4124041..4126191 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LNN46_RS19790 (4126241) 4126241..4126444 + 204 WP_000467859.1 YbdD/YjiX family protein -
LNN46_RS19795 (4126455) 4126455..4127411 + 957 WP_001297640.1 GTPase -
LNN46_RS19800 (4127445) 4127445..4127747 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LNN46_RS19805 (4127734) 4127734..4128021 - 288 WP_001297643.1 BrnT family toxin Toxin
LNN46_RS26375 (4128220) 4128220..4128916 - 697 Protein_3900 IS1 family transposase -
LNN46_RS19820 (4129231) 4129231..4130463 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
LNN46_RS19825 (4130504) 4130504..4131784 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4128220..4128429 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T223516 WP_001297643.1 NZ_CP086480:c4128021-4127734 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T223516 NZ_CP118932:4083583-4083927 [Serratia marcescens]
ATGTGGCAAGTGGTGACCGTCGAACGGTTTGACGACTGGTTTTTAGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTACAAATTGCAGACGTTCGGCCCGCAATTGGCGCGGCCGCATGCCGATACGCTGCATTTTTCCGACGCGGTCC
GGCAGTTGAAGGAGCTGCGCATTCAACATCGCGGGCGCCCTTTCAGGGTGTTTTTCGCCTTTGATCCACAGCGGCAAGCG
GTATTGCTGTGCGGCGGCGATAAAACCGGCGACAAACGCTTTTACCAGCGCATGTTGCCCATCGCCGCCATGGAGTTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT223516 WP_000063160.1 NZ_CP086480:c4127747-4127445 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT223516 NZ_CP118932:4083933-4084235 [Serratia marcescens]
ATGGCCAAACCTTTATCCCAACTGATCGATAAACTCGATCCCGCCGTCGTTGCCGCCGCCAGGCAAAAAGCGGACCAGGA
GATTTTCGAGCTGCGGCTGGCGATGCTGAGGGAAGAGTTGGCGGTTTCTCAGGTTGAATTGGCGAAGCGGCTCGGCATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATCAAACTGTCATCGCTCAAACGCTATATCGAGGCGATG
GGCGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAGCACCGCCGCATGACGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References