Detailed information of TA system TA223494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 25669..26134
• Replicon: plasmid pEc-050-T20-ESBL_6
• Accession: NZ_CP086476
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A5Y6UAV0
• Locus tag: LNN47_RS26070
• Protein ID: WP_001230708.1
• Coordinates: 25883..26134 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: LNN47_RS26065
• Protein ID: WP_000801440.1
• Coordinates: 25669..25896 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS26025 (LNN47_26030)	21039..21215	-	177	WP_000660496.1	hypothetical protein	-
LNN47_RS26030 (LNN47_26035)	21392..21607	-	216	WP_000760375.1	EexN family lipoprotein	-
LNN47_RS26035 (LNN47_26040)	21604..21897	-	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
LNN47_RS26040 (LNN47_26045)	21974..23203	-	1230	WP_000539545.1	DNA relaxase/nickase TaxC	-
LNN47_RS26045 (LNN47_26050)	23207..23710	-	504	WP_000781818.1	DNA distortion polypeptide 1	-
LNN47_RS26050 (LNN47_26055)	24073..24246	-	174	WP_071821486.1	type II toxin-antitoxin system HicB family antitoxin	-
LNN47_RS26055 (LNN47_26060)	24521..24886	-	366	WP_000854262.1	hypothetical protein	-
LNN47_RS26060 (LNN47_26065)	24978..25277	-	300	WP_000650313.1	hypothetical protein	-
LNN47_RS26065 (LNN47_26070)	25669..25896	-	228	WP_000801440.1	hypothetical protein	Antitoxin
LNN47_RS26070 (LNN47_26075)	25883..26134	-	252	WP_001230708.1	hypothetical protein	Toxin
LNN47_RS26075 (LNN47_26080)	26131..26673	-	543	WP_001083908.1	hypothetical protein	-
LNN47_RS26080 (LNN47_26085)	26778..27293	-	516	WP_001025396.1	J domain-containing protein	-
LNN47_RS26085 (LNN47_26090)	27932..28282	-	351	WP_000127483.1	hypothetical protein	-
LNN47_RS26090 (LNN47_26095)	28279..28614	-	336	WP_001147119.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33826	33826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (23-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9750.19 Da
• Isoelectric Point: 9.8104

>T223494 WP_001230708.1 NZ_CP086476:c26134-25883 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T223494 NZ_CP118916:c3098086-3097718 [Pseudoalteromonas ostreae]
TTGATAAGTGTACGTTATTTGCGTATATTTAATGGCATGAAATCTATCTTTATAGAATCGGTTATTTTTGAAAAATACCG
ACAACAGTATTTAAGTGATGAAGAGTATCGGCTTTTTCAAGCTGACCTAATGCGCAGTCCTAAACAAGGTGATGTAATTC
AGGGAACTGGCGGTTTACGTAAAATACGAGTTGCAGGTAAAGGGCGAGGAAAACGTGGTGGTTCAAGGGTTATCTATTAC
CATTTAGATGAATTTCAGCGTTTTTACCTACTGACCATTTACAGTAAAAATGAAATGACAGATTTAACAGCAGATCAAAA
GAAACAGTTAAAAGACTTTATGGAGGCGTGGCACAATGAGCAATCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT223494 WP_000801440.1 NZ_CP086476:c25896-25669 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT223494 NZ_CP118916:c3097731-3097417 [Pseudoalteromonas ostreae]
ATGAGCAATCGTGATTTATTTTCAGAGCTAAGTACAGCGCTTGTAGATGCTAAAGCACATTCGCAAGGTAAGCTAACCTT
ACGCACGCATGCTGTGAGTGACGCAAGTGATCTCGCTATTTCACCCGATGAAATAATCAATATTCGCGAAGCATTTAATA
TGTCACGCGGGGTATTTGCAAACCTATTACATACCTCAGCAAGAACCCTTGAAAACTGGGAGCAAGGCCGCAGCGCACCT
AATGGTCAAGCAATTACTTTACTAAAACTAGTGCAGCGTTATCCTGAGACTTTAAATCATATAGCTGAGTTGTAG

Structures

Toxin

• AlphaFold DB: A0A5Y6UAV0

Antitoxin

• AlphaFold DB: A0A5Y6U8W9