Detailed information of TA system TA223489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 78888..79489
• Replicon: plasmid pEc-050-T20-ESBL_3
• Accession: NZ_CP086473
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LNN47_RS24965
• Protein ID: WP_168420618.1
• Coordinates: 78888..79268 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: LNN47_RS24970
• Protein ID: WP_001190712.1
• Coordinates: 79268..79489 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS24935 (LNN47_24940)	74910..75938	+	1029	WP_168420624.1	tyrosine-type recombinase/integrase	-
LNN47_RS24940 (LNN47_24945)	76012..76356	+	345	WP_001191776.1	hypothetical protein	-
LNN47_RS24945 (LNN47_24950)	76353..76829	+	477	WP_000861174.1	hypothetical protein	-
LNN47_RS24950 (LNN47_24955)	76826..77320	+	495	WP_000640906.1	dUTP diphosphatase	-
LNN47_RS24955 (LNN47_24960)	77335..77997	+	663	WP_168420622.1	DUF2829 domain-containing protein	-
LNN47_RS24960 (LNN47_24965)	78004..78804	+	801	WP_168420620.1	hypothetical protein	-
LNN47_RS24965 (LNN47_24970)	78888..79268	-	381	WP_168420618.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LNN47_RS24970 (LNN47_24975)	79268..79489	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LNN47_RS24975 (LNN47_24980)	79562..79951	-	390	WP_168420616.1	S24 family peptidase	-
LNN47_RS24980 (LNN47_24985)	80075..80326	-	252	WP_063074807.1	DNA polymerase III subunit theta	-
LNN47_RS24985 (LNN47_24990)	80641..81399	-	759	WP_072756859.1	hypothetical protein	-
LNN47_RS24990 (LNN47_24995)	81396..81605	-	210	WP_075660655.1	hypothetical protein	-
LNN47_RS24995 (LNN47_25000)	81685..81861	-	177	WP_157902266.1	hypothetical protein	-
LNN47_RS25000 (LNN47_25005)	81861..82154	-	294	WP_075660654.1	hypothetical protein	-
LNN47_RS25005 (LNN47_25010)	82167..82571	-	405	WP_080077662.1	hypothetical protein	-
LNN47_RS25010 (LNN47_25015)	82564..82845	-	282	WP_102218741.1	ASCH domain-containing protein	-
LNN47_RS25015 (LNN47_25020)	82838..83029	-	192	WP_242806062.1	DUF551 domain-containing protein	-
LNN47_RS25020 (LNN47_25025)	83540..83665	-	126	Protein_97	DUF551 domain-containing protein	-
LNN47_RS25025 (LNN47_25030)	83662..83871	-	210	WP_000951710.1	hypothetical protein	-
LNN47_RS25030 (LNN47_25035)	83873..84067	-	195	WP_000224731.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89996	89996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13605.50 Da
• Isoelectric Point: 5.6406

>T223489 WP_168420618.1 NZ_CP086473:c79268-78888 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T223489 NZ_CP118907:c210626-210156 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT223489 WP_001190712.1 NZ_CP086473:c79489-79268 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT223489 NZ_CP118907:c211066-210623 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6