Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 75891..76416 Replicon plasmid pEc-050-T20-ESBL_2
Accession NZ_CP086472
Organism Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag LNN47_RS24395 Protein ID WP_001159871.1
Coordinates 75891..76196 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag LNN47_RS24400 Protein ID WP_000813630.1
Coordinates 76198..76416 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN47_RS24380 (71853) 71853..73019 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
LNN47_RS24385 (73607) 73607..74362 - 756 WP_000852146.1 replication initiation protein RepE -
LNN47_RS24390 (75084) 75084..75890 - 807 WP_000016970.1 site-specific integrase -
LNN47_RS24395 (75891) 75891..76196 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
LNN47_RS24400 (76198) 76198..76416 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LNN47_RS24405 (76976) 76976..77206 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
LNN47_RS24410 (77203) 77203..77619 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
LNN47_RS24415 (77694) 77694..79259 + 1566 WP_001128474.1 AAA family ATPase -
LNN47_RS24420 (79244) 79244..80266 + 1023 WP_000361402.1 helicase UvrD -
LNN47_RS24425 (80520) 80520..81206 - 687 Protein_100 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aac(3)-IIa / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..99465 99465


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T223486 WP_001159871.1 NZ_CP086472:c76196-75891 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T223486 NZ_CP118906:c5171167-5170784 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT223486 WP_000813630.1 NZ_CP086472:c76416-76198 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT223486 NZ_CP118906:c5171409-5171167 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References