Detailed information of TA system TA223477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1986..2533
• Replicon: plasmid pEc-050-T20-ESBL_1
• Accession: NZ_CP086471
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LNN47_RS23290
• Protein ID: WP_244442846.1
• Coordinates: 2255..2533 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: LNN47_RS23285
• Protein ID: WP_000079941.1
• Coordinates: 1986..2255 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS23275 (46)	46..1077	+	1032	WP_000907873.1	plasmid replication initiator RepA	-
LNN47_RS23280 (1773)	1773..1931	+	159	WP_000841008.1	hypothetical protein	-
LNN47_RS23285 (1986)	1986..2255	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
LNN47_RS23290 (2255)	2255..2533	+	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
LNN47_RS23295 (2573)	2573..3421	+	849	WP_001057996.1	3'-5' exonuclease	-
LNN47_RS23300 (3538)	3538..4011	+	474	WP_001334658.1	hypothetical protein	-
LNN47_RS23305 (4488)	4488..4754	-	267	WP_000955263.1	hypothetical protein	-
LNN47_RS23310 (4754)	4754..5248	-	495	WP_001459505.1	hypothetical protein	-
LNN47_RS23315 (5304)	5304..5906	-	603	WP_000517695.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / floR / blaTEM-30 / sul1 / qacE / ant(3'')-Ia / dfrA1	-	1..115932	115932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T223477 WP_244442846.1 NZ_CP086471:2255-2533 [Escherichia coli]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T223477 NZ_CP118906:327769-328137 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT223477 WP_000079941.1 NZ_CP086471:1986-2255 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT223477 NZ_CP118906:327551-327772 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5