Detailed information of TA system TA223470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2541567..2542205
• Replicon: chromosome
• Accession: NZ_CP086470
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: LNN47_RS12175
• Protein ID: WP_001447010.1
• Coordinates: 2542029..2542205 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LNN47_RS12170
• Protein ID: WP_001270286.1
• Coordinates: 2541567..2541983 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS12150 (2536719)	2536719..2537660	-	942	WP_001251304.1	ABC transporter permease	-
LNN47_RS12155 (2537661)	2537661..2538674	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LNN47_RS12160 (2538692)	2538692..2539837	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LNN47_RS12165 (2540082)	2540082..2541488	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LNN47_RS12170 (2541567)	2541567..2541983	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LNN47_RS12175 (2542029)	2542029..2542205	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LNN47_RS12180 (2542427)	2542427..2542657	+	231	WP_000494244.1	YncJ family protein	-
LNN47_RS12185 (2542749)	2542749..2544710	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LNN47_RS12190 (2544783)	2544783..2545319	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LNN47_RS12195 (2545411)	2545411..2546586	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T223470 WP_001447010.1 NZ_CP086470:c2542205-2542029 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T223470 NZ_CP118901:c4789657-4789373 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT223470 WP_001270286.1 NZ_CP086470:c2541983-2541567 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT223470 NZ_CP118901:c4789889-4789647 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8