Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933027..933681 Replicon chromosome
Accession NZ_CP086470
Organism Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LNN47_RS04585 Protein ID WP_000244777.1
Coordinates 933274..933681 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LNN47_RS04580 Protein ID WP_000354046.1
Coordinates 933027..933293 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN47_RS04555 (928196) 928196..928939 + 744 WP_000951964.1 SDR family oxidoreductase -
LNN47_RS04560 (928996) 928996..930429 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LNN47_RS04565 (930474) 930474..930785 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LNN47_RS04570 (930949) 930949..931608 + 660 WP_000250274.1 hemolysin III family protein -
LNN47_RS04575 (931804) 931804..932784 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LNN47_RS04580 (933027) 933027..933293 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LNN47_RS04585 (933274) 933274..933681 + 408 WP_000244777.1 protein YgfX Toxin
LNN47_RS04590 (933721) 933721..934242 - 522 WP_001055874.1 flavodoxin FldB -
LNN47_RS04595 (934354) 934354..935250 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LNN47_RS04600 (935275) 935275..935985 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LNN47_RS04605 (935991) 935991..937724 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T223457 WP_000244777.1 NZ_CP086470:933274-933681 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223457 NZ_CP118892:1960933-1961253 [Acinetobacter sp. TAC-1]
ATGTTATTCATTGAAACTAGTATTTTTACTAAACAAATCAAAGAGCTTGTGACTGATGATGAATATCGTCAGCTTCAACA
AAATCTATTGGTACAACCTGATAAGGGTGATATTGTTAAAAATGGCGGTGGTATCCGTAAAGTACGCTGTGCTCAAGGTG
ATAAAGGAAAAAGTGGTGGAATCCGCGTGATCTATTATTGGGTTTCAGAGGATGATCATATTTTCTTTTTAGTCGCGTAC
CCTAAGTCTGTAAAAGATAATTTAACGGATAAAGAAACAGCAATTCTGCGCCAATTAGTGAAGGAGCAACTTCATGGATA
A

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223457 WP_000354046.1 NZ_CP086470:933027-933293 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223457 NZ_CP118892:1961246-1961518 [Acinetobacter sp. TAC-1]
ATGGATAAAAATTTATTTGATGACCTTGTTTCTTCGATTAAAGAAGCAGGAGAGATTAAACGTCAAGAGATTCAAGCAAG
TCGAGTCACTGAGCTTGAATTACCTGATATTAAAGCAGTACGTGAGAAAACAGGTTTAAGCCAAAATGAATTTGCTGCAC
GCTTGCATATCAGTCCTCGCACGCTTCAAAACTGGGAACAAGGTAGACGTTATCCGACTGGTCCAGCTGCGACTTTAATC
CGTATTCTAGATACGCATCCAAGCCTAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References