Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5374233..5374858 Replicon chromosome
Accession NZ_CP086467
Organism Klebsiella pneumoniae strain MCL-2017-2-T0

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LNN43_RS26235 Protein ID WP_002882817.1
Coordinates 5374233..5374616 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LNN43_RS26240 Protein ID WP_004150355.1
Coordinates 5374616..5374858 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN43_RS26220 (5371599) 5371599..5372501 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LNN43_RS26225 (5372498) 5372498..5373133 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LNN43_RS26230 (5373130) 5373130..5374059 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LNN43_RS26235 (5374233) 5374233..5374616 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN43_RS26240 (5374616) 5374616..5374858 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LNN43_RS26245 (5375063) 5375063..5375980 + 918 WP_009484979.1 alpha/beta hydrolase -
LNN43_RS26250 (5375994) 5375994..5376935 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LNN43_RS26255 (5376980) 5376980..5377417 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LNN43_RS26260 (5377414) 5377414..5378274 - 861 WP_004146232.1 virulence factor BrkB family protein -
LNN43_RS26265 (5378268) 5378268..5378867 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T223451 WP_002882817.1 NZ_CP086467:c5374616-5374233 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T223451 NZ_CP118863:c4342211-4340892 [Providencia rettgeri]
ATGGCGACTTTGCATGTATACATGAATGGTTACTTGGTTGGGGATTTGATTCGTTCAAGTACTGGTGCTCACCAATTTAC
ATATAATAGTCACTGGTTGGAAACATCAGGTGCTCGACCTATTTCTTTATCAATGCCACTTAGGCACCAAGAATATCGAG
GAGACGAAGTTTATAATTTTTTTGATAATCTCCTACCGGACAATATTGAGATACGGAATCGAATTGTTAGTCGTTATCAA
GCGGATTCAAATCAACCTTTCGATTTATTATCTAAGATTGGTCAGGATAGTGTGGGGGCTTTACAACTCATTCTAGATGG
GCAAAGAGTCAGTAATATCAGAAAAATTGAATATAAACCTTTATCTAATAAGCAACTAGAAAAAATATTAACAGGGTATA
AAGCGAATGCTCCTTTAGGCATGATTGAAGATGAAGATGACTTTAGGATCTCACTTGCGGGCGCTCAAGAGAAAACCGCA
TTACTCTATTTAGATGGGCATTGGTGCTTACCTCAGAATGCGACTCCAACTACCCATATTATTAAACTTCCCATTGGTAA
AATAGAAAGCCATTCTTATTCGATAGATATGTCGGATAGTGTAGAAAATGAATATTTATGTTTATTGATAGCAAAAGAGT
TTGGCCTAGATGTGCCCTATAGCTTTATTTTAAATGTAGGTGAAATAAAGGCGTTAGCGGTTGAACGTTTTGACCGTAAA
TATTCTTCTGATTCCTCGTGGATTATGCGTTTACCACAAGAAGATTTTTGTCAAACATTGAATATTTCACCGGCAAGAAA
ATATCAAAATCATGGTGGACCGGGTATTTCTGAAATTATGAATTACTTATTAGGTTCAATAAATGCGGAGAAAGATAGAT
ATCAGTTTATGAAGAGCCAGGTTTTGTTTTGGTTACTTGCAGCGACGGATGGCCATGCTAAAAATTTTTCATTATTTATT
GAACCTGAAGGCCGTTATCAGTTAACACCTTTTTACGATATTTTGTCCATGTATCCTAGTTATGGGGGGAGGGGAATTAA
CCCTAGAGATGCCAAATTGGCTATGGGGTTGAAGGGAACAAGGGGGATGAAATACAATATAGAGCAAATTTTCCCAAGAC
ATTTTTTTTCAACGGCCAAAGCCGTTGGATTTGATCGTGCTGAGATGGAAAAAATCTTAATTGAATTTGGTGAGCAAATG
GAAAGTGTTATTAAGAAAGTGAGAGAACAATTACCCACAGATTTCCCTGAGCATATCGCAGACTCAATTCTATCCGGATT
ACATCACAAAGCAGCGAGATTAAATAAAGGCTGGGACTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT223451 WP_004150355.1 NZ_CP086467:c5374858-5374616 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT223451 NZ_CP118863:c4342468-4342211 [Providencia rettgeri]
ATGAAAGTGACGAATACCCGACAACTTAGTGCTTATATGAAGGATGTCAGAGAGATGAAAAAGTTATCTCAAAGCAAAGT
CGCTAGTAAAGTAGGTATTCGGCAAGATACTGTTTCTAGCTTTGAACTCTCTCCTGACTCTACCAAATTAGAAACCTTTT
TTAAGATTTTATCTGCCCTCAATTTAGAACTCGATATTAAACCGCGAAATGAAAGTGGCTCATCAAATTCAGACTCCGGA
TGGAAGGAGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References