Detailed information of TA system TA223450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5050269..5051950
• Replicon: chromosome
• Accession: NZ_CP086467
• Organism: Klebsiella pneumoniae strain MCL-2017-2-T0

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: LNN43_RS24730
• Protein ID: WP_002885155.1
• Coordinates: 5050269..5051186 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: LNN43_RS24735
• Protein ID: WP_004177809.1
• Coordinates: 5051192..5051950 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN43_RS24710 (5045575)	5045575..5047095	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
LNN43_RS24715 (5047092)	5047092..5048090	+	999	WP_002885172.1	ribose ABC transporter permease	-
LNN43_RS24720 (5048121)	5048121..5049062	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LNN43_RS24725 (5049137)	5049137..5050144	+	1008	WP_004177805.1	carbohydrate kinase family protein	-
LNN43_RS24730 (5050269)	5050269..5051186	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LNN43_RS24735 (5051192)	5051192..5051950	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LNN43_RS24740 (5052036)	5052036..5052764	-	729	WP_004177810.1	response regulator transcription factor	-
LNN43_RS24745 (5053037)	5053037..5055184	+	2148	WP_077598921.1	formate dehydrogenase subunit alpha	-
LNN43_RS24750 (5055414)	5055414..5056100	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T223450 WP_002885155.1 NZ_CP086467:c5051186-5050269 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T223450 NZ_CP118863:c4214544-4214242 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATTAAAAACCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGTGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAATAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTTCTTTTTGGTGAAGTTGAGGGGGAACTATGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT223450 WP_004177809.1 NZ_CP086467:c5051950-5051192 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT223450 NZ_CP118863:c4214245-4213961 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A6TH05