Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5374225..5374850 Replicon chromosome
Accession NZ_CP086464
Organism Klebsiella pneumoniae strain MCL-2017-2-T5-MAC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LNN59_RS26245 Protein ID WP_002882817.1
Coordinates 5374225..5374608 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LNN59_RS26250 Protein ID WP_004150355.1
Coordinates 5374608..5374850 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN59_RS26230 (5371591) 5371591..5372493 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LNN59_RS26235 (5372490) 5372490..5373125 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LNN59_RS26240 (5373122) 5373122..5374051 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LNN59_RS26245 (5374225) 5374225..5374608 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN59_RS26250 (5374608) 5374608..5374850 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LNN59_RS26255 (5375055) 5375055..5375972 + 918 WP_009484979.1 alpha/beta hydrolase -
LNN59_RS26260 (5375986) 5375986..5376927 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LNN59_RS26265 (5376972) 5376972..5377409 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LNN59_RS26270 (5377406) 5377406..5378266 - 861 WP_004146232.1 virulence factor BrkB family protein -
LNN59_RS26275 (5378260) 5378260..5378859 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T223431 WP_002882817.1 NZ_CP086464:c5374608-5374225 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T223431 NZ_CP118842:994430-994609 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT223431 WP_004150355.1 NZ_CP086464:c5374850-5374608 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT223431 NZ_CP118842:993807-994406 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References