Detailed information of TA system TA223430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5050257..5051938
• Replicon: chromosome
• Accession: NZ_CP086464
• Organism: Klebsiella pneumoniae strain MCL-2017-2-T5-MAC

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: LNN59_RS24740
• Protein ID: WP_002885155.1
• Coordinates: 5050257..5051174 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: LNN59_RS24745
• Protein ID: WP_004177809.1
• Coordinates: 5051180..5051938 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN59_RS24720 (5045563)	5045563..5047083	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
LNN59_RS24725 (5047080)	5047080..5048078	+	999	WP_002885172.1	ribose ABC transporter permease	-
LNN59_RS24730 (5048109)	5048109..5049050	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LNN59_RS24735 (5049125)	5049125..5050132	+	1008	WP_004177805.1	carbohydrate kinase family protein	-
LNN59_RS24740 (5050257)	5050257..5051174	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LNN59_RS24745 (5051180)	5051180..5051938	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LNN59_RS24750 (5052024)	5052024..5052752	-	729	WP_004177810.1	response regulator transcription factor	-
LNN59_RS24755 (5053025)	5053025..5055172	+	2148	WP_077598921.1	formate dehydrogenase subunit alpha	-
LNN59_RS24760 (5055402)	5055402..5056088	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T223430 WP_002885155.1 NZ_CP086464:c5051174-5050257 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T223430 NZ_CP118842:845414-845776 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT223430 WP_004177809.1 NZ_CP086464:c5051938-5051180 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT223430 NZ_CP118842:845247-845417 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A6TH05