Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4124708..4125327 Replicon chromosome
Accession NZ_CP086464
Organism Klebsiella pneumoniae strain MCL-2017-2-T5-MAC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LNN59_RS20330 Protein ID WP_002892050.1
Coordinates 4125109..4125327 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LNN59_RS20325 Protein ID WP_002892066.1
Coordinates 4124708..4125082 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN59_RS20315 (4119860) 4119860..4121053 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN59_RS20320 (4121076) 4121076..4124222 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LNN59_RS20325 (4124708) 4124708..4125082 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LNN59_RS20330 (4125109) 4125109..4125327 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LNN59_RS20335 (4125486) 4125486..4126052 + 567 WP_002892030.1 maltose O-acetyltransferase -
LNN59_RS20340 (4126024) 4126024..4126164 - 141 WP_004147370.1 hypothetical protein -
LNN59_RS20345 (4126185) 4126185..4126655 + 471 WP_002892026.1 YlaC family protein -
LNN59_RS20350 (4126630) 4126630..4128081 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
LNN59_RS20355 (4128182) 4128182..4128880 + 699 WP_032435564.1 GNAT family protein -
LNN59_RS20360 (4128877) 4128877..4129017 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LNN59_RS20365 (4129017) 4129017..4129280 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223426 WP_002892050.1 NZ_CP086464:4125109-4125327 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T223426 NZ_CP118835:c2511558-2511292 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT223426 WP_002892066.1 NZ_CP086464:4124708-4125082 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT223426 NZ_CP118835:c2511815-2511558 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGGTAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References