Detailed information of TA system TA223409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4889805..4890321
• Replicon: chromosome
• Accession: NZ_CP086461
• Organism: Klebsiella pneumoniae strain MCL-2017-2-T5-ESBL

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: LNN56_RS23930
• Protein ID: WP_004178374.1
• Coordinates: 4889805..4890089 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: LNN56_RS23935
• Protein ID: WP_032434351.1
• Coordinates: 4890079..4890321 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN56_RS23905 (4885288)	4885288..4885551	-	264	WP_014908078.1	PTS sugar transporter subunit IIB	-
LNN56_RS23910 (4885681)	4885681..4885854	+	174	WP_032414379.1	hypothetical protein	-
LNN56_RS23915 (4885857)	4885857..4886600	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
LNN56_RS23920 (4886957)	4886957..4889095	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
LNN56_RS23925 (4889337)	4889337..4889801	+	465	WP_004222151.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LNN56_RS23930 (4889805)	4889805..4890089	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN56_RS23935 (4890079)	4890079..4890321	-	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LNN56_RS23940 (4890399)	4890399..4892309	-	1911	WP_004152270.1	PRD domain-containing protein	-
LNN56_RS23945 (4892332)	4892332..4893486	-	1155	WP_002886900.1	lactonase family protein	-
LNN56_RS23950 (4893552)	4893552..4894292	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T223409 WP_004178374.1 NZ_CP086461:c4890089-4889805 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T223409 NZ_CP118832:c2123660-2123298 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT223409 WP_032434351.1 NZ_CP086461:c4890321-4890079 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT223409 NZ_CP118832:c2123827-2123657 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A6THG1