Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4766412..4767222 Replicon chromosome
Accession NZ_CP086461
Organism Klebsiella pneumoniae strain MCL-2017-2-T5-ESBL

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LNN56_RS23405 Protein ID WP_004178461.1
Coordinates 4766412..4766945 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LNN56_RS23410 Protein ID WP_002887278.1
Coordinates 4766956..4767222 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN56_RS23400 (4765243) 4765243..4766364 + 1122 WP_004186850.1 cupin domain-containing protein -
LNN56_RS23405 (4766412) 4766412..4766945 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LNN56_RS23410 (4766956) 4766956..4767222 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LNN56_RS23415 (4767325) 4767325..4768758 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
LNN56_RS23420 (4768748) 4768748..4769431 - 684 WP_231547709.1 copper response regulator transcription factor CusR -
LNN56_RS23425 (4769604) 4769604..4770989 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
LNN56_RS23430 (4771007) 4771007..4771351 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T223407 WP_004178461.1 NZ_CP086461:c4766945-4766412 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T223407 NZ_CP118832:c2026857-2026681 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT223407 WP_002887278.1 NZ_CP086461:c4767222-4766956 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT223407 NZ_CP118832:2026550-2026691 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References