Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4124711..4125330 Replicon chromosome
Accession NZ_CP086461
Organism Klebsiella pneumoniae strain MCL-2017-2-T5-ESBL

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LNN56_RS20325 Protein ID WP_002892050.1
Coordinates 4125112..4125330 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LNN56_RS20320 Protein ID WP_002892066.1
Coordinates 4124711..4125085 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN56_RS20310 (4119863) 4119863..4121056 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN56_RS20315 (4121079) 4121079..4124225 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LNN56_RS20320 (4124711) 4124711..4125085 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LNN56_RS20325 (4125112) 4125112..4125330 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LNN56_RS20330 (4125489) 4125489..4126055 + 567 WP_002892030.1 maltose O-acetyltransferase -
LNN56_RS20335 (4126027) 4126027..4126167 - 141 WP_004147370.1 hypothetical protein -
LNN56_RS20340 (4126188) 4126188..4126658 + 471 WP_002892026.1 YlaC family protein -
LNN56_RS20345 (4126633) 4126633..4128084 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
LNN56_RS20350 (4128185) 4128185..4128883 + 699 WP_032435564.1 GNAT family protein -
LNN56_RS20355 (4128880) 4128880..4129020 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LNN56_RS20360 (4129020) 4129020..4129283 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223406 WP_002892050.1 NZ_CP086461:4125112-4125330 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T223406 NZ_CP118832:c1925846-1925694 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT223406 WP_002892066.1 NZ_CP086461:4124711-4125085 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT223406 NZ_CP118832:c1926470-1925871 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References