Detailed information of TA system TA223392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53674..54410
• Replicon: plasmid pMCL-2017-T20-MAC_1
• Accession: NZ_CP086459
• Organism: Klebsiella pneumoniae strain MCL-2017-2-T20-MAC

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LNN57_RS26895
• Protein ID: WP_003026803.1
• Coordinates: 53928..54410 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LNN57_RS26890
• Protein ID: WP_003026799.1
• Coordinates: 53674..53940 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN57_RS26845 (LNN57_26845)	49736..50098	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
LNN57_RS26850 (LNN57_26850)	50148..50498	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
LNN57_RS26855 (LNN57_26855)	50856..51125	+	270	WP_004152102.1	hypothetical protein	-
LNN57_RS26860 (LNN57_26860)	51113..51688	+	576	WP_004152103.1	hypothetical protein	-
LNN57_RS26865 (LNN57_26865)	51719..52213	+	495	WP_004152104.1	DNA-binding protein	-
LNN57_RS26870 (LNN57_26870)	52257..52625	+	369	WP_004152105.1	hypothetical protein	-
LNN57_RS26875 (LNN57_26875)	52659..52862	+	204	WP_004152106.1	HHA domain-containing protein	-
LNN57_RS26880 (LNN57_26880)	52911..53168	+	258	WP_004152107.1	hypothetical protein	-
LNN57_RS26885 (LNN57_26885)	53244..53498	+	255	WP_004152108.1	hypothetical protein	-
LNN57_RS26890 (LNN57_26890)	53674..53940	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LNN57_RS26895 (LNN57_26895)	53928..54410	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LNN57_RS26900 (LNN57_26900)	54618..55964	+	1347	WP_077253535.1	ISNCY family transposase	-
LNN57_RS26905 (LNN57_26905)	56013..56408	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
LNN57_RS26910 (LNN57_26910)	56556..57721	-	1166	Protein_59	IS3 family transposase	-
LNN57_RS26915 (LNN57_26915)	57898..58860	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
LNN57_RS26920 (LNN57_26920)	58847..59335	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa	-	1..247089	247089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T223392 WP_003026803.1 NZ_CP086459:53928-54410 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T223392 NZ_CP118830:1824051-1824146 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT223392 WP_003026799.1 NZ_CP086459:53674-53940 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT223392 NZ_CP118830:c1824233-1824174 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66