Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4766401..4767211 Replicon chromosome
Accession NZ_CP086458
Organism Klebsiella pneumoniae strain MCL-2017-2-T20-MAC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LNN57_RS23415 Protein ID WP_004178461.1
Coordinates 4766401..4766934 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LNN57_RS23420 Protein ID WP_002887278.1
Coordinates 4766945..4767211 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN57_RS23410 (4765232) 4765232..4766353 + 1122 WP_004186850.1 cupin domain-containing protein -
LNN57_RS23415 (4766401) 4766401..4766934 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LNN57_RS23420 (4766945) 4766945..4767211 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LNN57_RS23425 (4767314) 4767314..4768747 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
LNN57_RS23430 (4768737) 4768737..4769420 - 684 WP_231547709.1 copper response regulator transcription factor CusR -
LNN57_RS23435 (4769593) 4769593..4770978 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
LNN57_RS23440 (4770996) 4770996..4771340 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T223387 WP_004178461.1 NZ_CP086458:c4766934-4766401 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T223387 NZ_CP118820:878893-879102 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATACATGTTTCAGGTATTAAAACAACTGAACAAGAGAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCCGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTGCGGATTTCGTATTTTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT223387 WP_002887278.1 NZ_CP086458:c4767211-4766945 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT223387 NZ_CP118820:878593-878886 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATCAATCGTATGATTGATGACGATGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTATTAAATTACTAGATCATCACATGAAGGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References