Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4124705..4125324 Replicon chromosome
Accession NZ_CP086458
Organism Klebsiella pneumoniae strain MCL-2017-2-T20-MAC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LNN57_RS20335 Protein ID WP_002892050.1
Coordinates 4125106..4125324 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LNN57_RS20330 Protein ID WP_002892066.1
Coordinates 4124705..4125079 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN57_RS20320 (4119857) 4119857..4121050 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN57_RS20325 (4121073) 4121073..4124219 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LNN57_RS20330 (4124705) 4124705..4125079 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LNN57_RS20335 (4125106) 4125106..4125324 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LNN57_RS20340 (4125483) 4125483..4126049 + 567 WP_002892030.1 maltose O-acetyltransferase -
LNN57_RS20345 (4126021) 4126021..4126161 - 141 WP_004147370.1 hypothetical protein -
LNN57_RS20350 (4126182) 4126182..4126652 + 471 WP_002892026.1 YlaC family protein -
LNN57_RS20355 (4126627) 4126627..4128078 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
LNN57_RS20360 (4128179) 4128179..4128877 + 699 WP_032435564.1 GNAT family protein -
LNN57_RS20365 (4128874) 4128874..4129014 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LNN57_RS20370 (4129014) 4129014..4129277 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223386 WP_002892050.1 NZ_CP086458:4125106-4125324 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T223386 NZ_CP118814:1956363-1956551 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT223386 WP_002892066.1 NZ_CP086458:4124705-4125079 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT223386 NZ_CP118814:1956081-1956338 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References