Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5374232..5374857 Replicon chromosome
Accession NZ_CP086455
Organism Klebsiella pneumoniae strain MCL-2017-2-T20-ESBL

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LNN48_RS26245 Protein ID WP_002882817.1
Coordinates 5374232..5374615 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LNN48_RS26250 Protein ID WP_004150355.1
Coordinates 5374615..5374857 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN48_RS26230 (5371598) 5371598..5372500 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LNN48_RS26235 (5372497) 5372497..5373132 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LNN48_RS26240 (5373129) 5373129..5374058 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LNN48_RS26245 (5374232) 5374232..5374615 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN48_RS26250 (5374615) 5374615..5374857 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LNN48_RS26255 (5375062) 5375062..5375979 + 918 WP_009484979.1 alpha/beta hydrolase -
LNN48_RS26260 (5375993) 5375993..5376934 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LNN48_RS26265 (5376979) 5376979..5377416 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LNN48_RS26270 (5377413) 5377413..5378273 - 861 WP_004146232.1 virulence factor BrkB family protein -
LNN48_RS26275 (5378267) 5378267..5378866 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T223371 WP_002882817.1 NZ_CP086455:c5374615-5374232 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T223371 NZ_CP118774:c4876537-4876163 [Citrobacter braakii]
ATGGTCACAGGATCTGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGCGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATCGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT223371 WP_004150355.1 NZ_CP086455:c5374857-5374615 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT223371 NZ_CP118774:c4876779-4876537 [Citrobacter braakii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCTGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References