Detailed information of TA system TA223369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4889807..4890323
• Replicon: chromosome
• Accession: NZ_CP086455
• Organism: Klebsiella pneumoniae strain MCL-2017-2-T20-ESBL

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: LNN48_RS23935
• Protein ID: WP_004178374.1
• Coordinates: 4889807..4890091 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: LNN48_RS23940
• Protein ID: WP_032434351.1
• Coordinates: 4890081..4890323 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN48_RS23910 (4885290)	4885290..4885553	-	264	WP_014908078.1	PTS sugar transporter subunit IIB	-
LNN48_RS23915 (4885683)	4885683..4885856	+	174	WP_032414379.1	hypothetical protein	-
LNN48_RS23920 (4885859)	4885859..4886602	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
LNN48_RS23925 (4886959)	4886959..4889097	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
LNN48_RS23930 (4889339)	4889339..4889803	+	465	WP_004222151.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LNN48_RS23935 (4889807)	4889807..4890091	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN48_RS23940 (4890081)	4890081..4890323	-	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LNN48_RS23945 (4890401)	4890401..4892311	-	1911	WP_004152270.1	PRD domain-containing protein	-
LNN48_RS23950 (4892334)	4892334..4893488	-	1155	WP_002886900.1	lactonase family protein	-
LNN48_RS23955 (4893554)	4893554..4894294	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T223369 WP_004178374.1 NZ_CP086455:c4890091-4889807 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T223369 NZ_CP118774:c4262960-4262589 [Citrobacter braakii]
ATGCAAACCATATCATCACACCCGACACGGGCGGCTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGTTGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTGATGAAGCCACCATCCAGGAACATATTG
ATGCAGGAATATCGCTTAGCGATGCGGTAAACTTTCTTGTCGAAAAATATGAACTGGTAAGAATTGACTGCGATGGTTGC
TCTGTCATGGAGAAGTCACCGTTTATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCAACATCAGGAGCTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT223369 WP_032434351.1 NZ_CP086455:c4890323-4890081 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT223369 NZ_CP118774:c4263364-4262999 [Citrobacter braakii]
ATGAACAATCACTCAGAATCCGGTGCCATACTTGAGAATTCCCCCTGCCAGCAGTGGGGGCTAAAGAGTACGATCACACC
CTGTTTTGGAGCTCGGCTGGTGCAAGAAGGCGACCGCCTGCATTTTCTCGCTGACCGCGCCGGGTTTAACGGTGCTTTCA
GTGACGAGCATGCGTTGCGCCTCGACCAGGCCTTTCCGTTAATACTCAAGCAGCTGGAGCTGATGCTCACCAGCGGAGAG
CTCAACCCTAGGCACCCGCACAGCGTCACGCTTTACCACAACGGACTCACCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTTCATTGCTATCTATCCTGAGCATACTGAGCCGCAGTAA

Structures

Toxin

• AlphaFold DB: A6THG1