Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4124710..4125329 Replicon chromosome
Accession NZ_CP086455
Organism Klebsiella pneumoniae strain MCL-2017-2-T20-ESBL

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LNN48_RS20330 Protein ID WP_002892050.1
Coordinates 4125111..4125329 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LNN48_RS20325 Protein ID WP_002892066.1
Coordinates 4124710..4125084 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN48_RS20315 (4119862) 4119862..4121055 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LNN48_RS20320 (4121078) 4121078..4124224 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LNN48_RS20325 (4124710) 4124710..4125084 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LNN48_RS20330 (4125111) 4125111..4125329 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LNN48_RS20335 (4125488) 4125488..4126054 + 567 WP_002892030.1 maltose O-acetyltransferase -
LNN48_RS20340 (4126026) 4126026..4126166 - 141 WP_004147370.1 hypothetical protein -
LNN48_RS20345 (4126187) 4126187..4126657 + 471 WP_002892026.1 YlaC family protein -
LNN48_RS20350 (4126632) 4126632..4128083 - 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
LNN48_RS20355 (4128184) 4128184..4128882 + 699 WP_032435564.1 GNAT family protein -
LNN48_RS20360 (4128879) 4128879..4129019 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LNN48_RS20365 (4129019) 4129019..4129282 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223366 WP_002892050.1 NZ_CP086455:4125111-4125329 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T223366 NZ_CP118774:2331233-2331421 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTACCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGTTTACAGTCATCATCAATATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT223366 WP_002892066.1 NZ_CP086455:4124710-4125084 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT223366 NZ_CP118774:2331453-2331869 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCGCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
CCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCACTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACAATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References