Detailed information of TA system TA223356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2309162..2310750
• Replicon: chromosome
• Accession: NZ_CP086455
• Organism: Klebsiella pneumoniae strain MCL-2017-2-T20-ESBL

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9J6RYF8
• Locus tag: LNN48_RS11270
• Protein ID: WP_004892016.1
• Coordinates: 2309428..2310750 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LNN48_RS11265
• Protein ID: WP_004176198.1
• Coordinates: 2309162..2309428 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN48_RS11240 (2304191)	2304191..2305114	+	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-
LNN48_RS11245 (2305095)	2305095..2306249	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LNN48_RS11250 (2306595)	2306595..2307974	+	1380	WP_004184021.1	nucleobase:cation symporter-2 family protein	-
LNN48_RS11255 (2307952)	2307952..2308452	+	501	WP_032435339.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LNN48_RS11260 (2308449)	2308449..2308775	+	327	WP_004184023.1	hydroxyisourate hydrolase	-
LNN48_RS11265 (2309162)	2309162..2309428	+	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LNN48_RS11270 (2309428)	2309428..2310750	+	1323	WP_004892016.1	type II toxin-antitoxin system HipA family toxin	Toxin
LNN48_RS11275 (2310767)	2310767..2312059	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LNN48_RS11280 (2312201)	2312201..2313205	-	1005	WP_032435338.1	fimbrial protein	-
LNN48_RS11285 (2313193)	2313193..2315694	-	2502	WP_032435337.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T223356 WP_004892016.1 NZ_CP086455:2309428-2310750 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T223356 NZ_CP118755:c2755874-2755533 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT223356 WP_004176198.1 NZ_CP086455:2309162-2309428 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT223356 NZ_CP118755:c2756104-2755874 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA