Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20837..21480 Replicon plasmid p2247421-T0_3
Accession NZ_CP086450
Organism Klebsiella pneumoniae strain AE-2247421-T0

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LNN40_RS28840 Protein ID WP_001044770.1
Coordinates 20837..21253 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LNN40_RS28845 Protein ID WP_001261282.1
Coordinates 21250..21480 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN40_RS28805 (LNN40_28810) 16379..16615 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
LNN40_RS28810 (LNN40_28815) 16612..16974 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
LNN40_RS28815 (LNN40_28820) 16992..18686 - 1695 WP_020442400.1 mercury(II) reductase -
LNN40_RS28820 (LNN40_28825) 18738..19160 - 423 WP_001340589.1 organomercurial transporter MerC -
LNN40_RS28825 (LNN40_28830) 19196..19306 - 111 Protein_22 mercuric transport protein periplasmic component -
LNN40_RS28835 (LNN40_28840) 20074..20763 - 690 Protein_24 AAA family ATPase -
LNN40_RS28840 (LNN40_28845) 20837..21253 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN40_RS28845 (LNN40_28850) 21250..21480 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LNN40_RS28850 (LNN40_28855) 21437..21898 + 462 WP_072202616.1 hypothetical protein -
LNN40_RS28855 (LNN40_28860) 22042..22392 + 351 WP_000493378.1 hypothetical protein -
LNN40_RS28860 (LNN40_28865) 22443..23186 + 744 WP_000129823.1 hypothetical protein -
LNN40_RS28865 (LNN40_28870) 23183..23959 + 777 WP_000015958.1 site-specific integrase -
LNN40_RS28870 (LNN40_28875) 24017..24274 - 258 WP_000764642.1 hypothetical protein -
LNN40_RS28875 (LNN40_28880) 24403..24507 - 105 WP_032409716.1 hypothetical protein -
LNN40_RS28880 (LNN40_28885) 25042..25908 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-15 / blaTEM-1A / dfrA14 - 1..71289 71289


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T223352 WP_001044770.1 NZ_CP086450:c21253-20837 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T223352 NZ_CP118755:c387695-387600 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT223352 WP_001261282.1 NZ_CP086450:c21480-21250 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT223352 NZ_CP118755:387501-387565 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References