Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53667..54403 Replicon plasmid p2247421-T0_2
Accession NZ_CP086449
Organism Klebsiella pneumoniae strain AE-2247421-T0

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LNN40_RS28125 Protein ID WP_003026803.1
Coordinates 53921..54403 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LNN40_RS28120 Protein ID WP_003026799.1
Coordinates 53667..53933 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN40_RS28075 (LNN40_28080) 49729..50091 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
LNN40_RS28080 (LNN40_28085) 50141..50491 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LNN40_RS28085 (LNN40_28090) 50849..51118 + 270 WP_004152102.1 hypothetical protein -
LNN40_RS28090 (LNN40_28095) 51106..51681 + 576 WP_004152103.1 hypothetical protein -
LNN40_RS28095 (LNN40_28100) 51712..52206 + 495 WP_004152104.1 DNA-binding protein -
LNN40_RS28100 (LNN40_28105) 52250..52618 + 369 WP_004152105.1 hypothetical protein -
LNN40_RS28105 (LNN40_28110) 52652..52855 + 204 WP_004152106.1 HHA domain-containing protein -
LNN40_RS28110 (LNN40_28115) 52904..53161 + 258 WP_004152107.1 hypothetical protein -
LNN40_RS28115 (LNN40_28120) 53237..53491 + 255 WP_004152108.1 hypothetical protein -
LNN40_RS28120 (LNN40_28125) 53667..53933 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LNN40_RS28125 (LNN40_28130) 53921..54403 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LNN40_RS28130 (LNN40_28135) 54604..56007 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LNN40_RS28135 (LNN40_28140) 56036..56668 - 633 WP_001567369.1 hypothetical protein -
LNN40_RS28140 (LNN40_28145) 56898..58244 + 1347 WP_020314316.1 ISNCY family transposase -
LNN40_RS29710 58405..58536 + 132 WP_004218042.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSCO-1 / aac(3)-IIa / blaTEM-1B - 1..158444 158444


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T223351 WP_003026803.1 NZ_CP086449:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T223351 NZ_CP118755:c143979-143341 [Enterococcus faecalis]
ATGATACTTAATAAGAAAAAATTATATTTCGAAACACAGGCATTTACTGACAAACTTATAAATGAAATTTCAATCAATAT
GTCTAAAAGAAAAGAACTCATCAATTGTTATGATATTGAAGCCTACGTAAAAGAAACCGAAAACATTGATTTTTTTGATT
ATCCATTCAAAAAACAATCTAAAAAATTAATCTGGGGATCAGTAACAAAAATTGGTGATGTAACTATGGTAGCGACTGCC
CAACACTTGAAAATAGAAATTAAAAATTTTACGAAAATGCACGAAATAATTCATCATTATTTTGACTATACGACAGATAT
ACCTAACAATGAAAAATCATTTGATACACTACTTATAAAAAAGGGCTATCTTCCTGAAGATTATCCTAAAGAATTCAGAG
CAAATAAGGGTGCCGGAATGTTACTTATTAATAAAAAAGCCTTAAAGTATGCATTTAGTACTTATGATGATCTTGAAAAA
ATGGCTAGTTTTTTCAACGTAAACGATATAGTTCTCAAGATAAGAATTCTTGACGAATTAATCTATTCCCACAACTTATC
TTATAAACAAGCCAATCTTATTTATAATAGTTACTATTTTCAACAACAAACACAACTTAAAGAGATTTTATTCAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT223351 WP_003026799.1 NZ_CP086449:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT223351 NZ_CP118755:c144360-143986 [Enterococcus faecalis]
ATGAAAACTTACGAAAAGATAAAACAATTAAGAGAAAATAGAGAAATCACTCAAAAAGAAATTTCAAACGCACTAGACAT
TAATGTTAGTGTATATAATAAAATAGAACTTGGAATACGTCCATTAAGAGAAGAAGAACTTACTGCTATTGCTGATTTTT
TTAATGTTTCAATTGATTATTTAACAGGACGCACTGATAACCCTACAACACCTTCAAATAATCAAAAAGAAGCTGGACAA
AATATTATTTCACATTTCAGATTAAACACTAATGATTTAAGTTCTGATGACATAGAAGAATTAGAAAAAGAATTGATTGA
TTTTCAAGAATTTCTTATAAAACGAGCAAAAGAAAGAAAAAAAAAGAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References