Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5386410..5387035 Replicon chromosome
Accession NZ_CP086447
Organism Klebsiella pneumoniae strain AE-2247421-T0

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LNN40_RS26385 Protein ID WP_002882817.1
Coordinates 5386410..5386793 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LNN40_RS26390 Protein ID WP_004150355.1
Coordinates 5386793..5387035 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN40_RS26370 (5383776) 5383776..5384678 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LNN40_RS26375 (5384675) 5384675..5385310 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LNN40_RS26380 (5385307) 5385307..5386236 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LNN40_RS26385 (5386410) 5386410..5386793 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LNN40_RS26390 (5386793) 5386793..5387035 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LNN40_RS26395 (5387240) 5387240..5388157 + 918 WP_032418237.1 alpha/beta hydrolase -
LNN40_RS26400 (5388171) 5388171..5389112 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LNN40_RS26405 (5389157) 5389157..5389594 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LNN40_RS26410 (5389591) 5389591..5390451 - 861 WP_004146232.1 virulence factor BrkB family protein -
LNN40_RS26415 (5390445) 5390445..5391044 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T223349 WP_002882817.1 NZ_CP086447:c5386793-5386410 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T223349 NZ_CP118744:c1428946-1428200 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTATTGGCGCTGGCTCTGTATGTGGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACATGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCCTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCATATGCAGAAGGGATCGAAAAGCTCATCAAACGATTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGCTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGACGGTGATGACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT223349 WP_004150355.1 NZ_CP086447:c5387035-5386793 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT223349 NZ_CP118744:c1428200-1428027 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAAGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References