Detailed information of TA system TA223335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1841562..1842152
• Replicon: chromosome
• Accession: NZ_CP086447
• Organism: Klebsiella pneumoniae strain AE-2247421-T0

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LNN40_RS09015
• Protein ID: WP_023341911.1
• Coordinates: 1841820..1842152 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: LNN40_RS09010
• Protein ID: WP_000288812.1
• Coordinates: 1841562..1841819 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN40_RS08985 (1837472)	1837472..1837546	+	75	Protein_1766	type II toxin-antitoxin system PemK/MazF family toxin	-
LNN40_RS08990 (1837897)	1837897..1839645	+	1749	WP_032418698.1	hypothetical protein	-
LNN40_RS08995 (1839699)	1839699..1840280	+	582	WP_099184326.1	hypothetical protein	-
LNN40_RS09000 (1840563)	1840563..1841024	+	462	WP_004213450.1	hypothetical protein	-
LNN40_RS09005 (1841021)	1841021..1841227	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
LNN40_RS09010 (1841562)	1841562..1841819	+	258	WP_000288812.1	antitoxin	Antitoxin
LNN40_RS09015 (1841820)	1841820..1842152	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LNN40_RS09025 (1842474)	1842474..1843910	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LNN40_RS09035 (1844276)	1844276..1845730	-	1455	WP_004148975.1	AMP nucleosidase	-
LNN40_RS09040 (1845860)	1845860..1846104	-	245	Protein_1775	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T223335 WP_023341911.1 NZ_CP086447:1841820-1842152 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T223335 NZ_CP118713:c3918824-3918534 [Rhizobium sp. BJ04]
ATGAAAACGCTTGCTCTCTCGCCAGCCGCTGCCGACGATATCGACAGGATTTATGACTATACCGAAGAGAAATGGGGGCA
GGATCAAGCGGAGGACTATATCCTTGCACTCCGGGATGACTGCGGGGCGCTTGCAGCACAGACAAAGCGTGGCCGCAAAA
TCATTGGATTGAGGAGCGGCTACACCGCTCTGGCGTTTCGCTCGCATTTCATCATTTACGCTGATACCGAAGAAGCGTTG
ATAGTTATTCGCATCCTGCATCGGCGCATGAATATCGCGGCCCATCTCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT223335 WP_000288812.1 NZ_CP086447:1841562-1841819 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT223335 NZ_CP118713:c3919066-3918821 [Rhizobium sp. BJ04]
GTGGCCAAGGTCGTGTCCATCAGAATCGATGATCACATGGAAGAATTTATCGGCAATCAGCTCGATCAGGGAACTTATGG
CTCTGCAGACGAGGTGATCGACGCCGGTTTGAGGCTTTTGCAGCGCGAGGCGGAGCTTGCAGCAATCGAGGCAGCCATTA
TCGACGGTGAGAAATCCGGCAAGCCACGGCCTTTTGATTTTGATGCTTTTATGGAGGGGAAGCGAGCACGCCGCAGCCGC
CAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0